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Rabbit抗原名称
MMP9分子别名
Matrix metalloproteinase-9; 92 kDa gelatinase; 92 kDa type IV collagenase; Gelatinase B (GELB); CLG4B免疫原
Synthetic Peptide细胞定位
SecretedAccession
P14780克隆号
S-3915-36抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt阳性样本
A549 (starved overnight, then treated with 100 ng/ml TPA for 24 hours, together with 1 µg/ml BFA for last 3 hours), mouse spleen, mouse lung, mouse bone marrow, rat spleen, rat lung纯化方式
Protein A浓度
0.25 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:500-1:1000 Hu, Ms, Rt IHC-P 1:2000 Hu, Ms, Rt
Matrix metalloproteinase-9 (MMP-9), also known as gelatinase B, is a zinc-dependent endopeptidase belonging to the matrix metalloproteinase family that plays a pivotal role in the degradation and remodeling of the extracellular matrix (ECM), particularly by cleaving type IV and V collagens, gelatin, elastin, and various non-matrix proteins such as cytokines and growth factors. Synthesized primarily by neutrophils, macrophages, fibroblasts, and epithelial cells as an inactive zymogen (pro-MMP-9), it requires proteolytic activation to exert its enzymatic function, a process tightly regulated by tissue inhibitors of metalloproteinases (TIMPs) to prevent excessive tissue destruction. Under physiological conditions, MMP-9 is essential for normal processes including embryonic development, wound healing, angiogenesis, and immune cell migration; however, its dysregulated overexpression is strongly implicated in numerous pathological states, such as tumor invasion and metastasis where it facilitates cancer cell penetration through basement membranes, as well as in inflammatory diseases like rheumatoid arthritis, multiple sclerosis, and cardiovascular disorders where it contributes to plaque instability and tissue damage, making it a significant biomarker and a potential therapeutic target in clinical research.
免疫印迹
WB result of MMP9 Recombinant Rabbit mAb
Primary antibody: MMP9 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated A549 whole cell lysate 20 µg
Lane 2: A549 starved overnight, then treated with 100 ng/ml TPA for 24 hours, together with 1 µg/ml BFA for last 3 hours whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 78 kDa
Observed MW: 78, 100 kDaWB result of MMP9 Recombinant Rabbit mAb
Primary antibody: MMP9 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse liver lysate 20 µg
Lane 2: mouse spleen lysate 20 µg
Lane 3: mouse lung lysate 20 µg
Lane 4: mouse bone marrow lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 78 kDa
Observed MW: 78, 110 kDaWB result of MMP9 Recombinant Rabbit mAb
Primary antibody: MMP9 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat spleen lysate 20 µg
Lane 2: rat lung lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 78 kDa
Observed MW: 78, 110 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human spleen. Anti-MMP9 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human tonsil. Anti-MMP9 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human skeletal muscle. Anti-MMP9 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse spleen. Anti-MMP9 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse lung. Anti-MMP9 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat spleen. Anti-MMP9 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat lung. Anti-MMP9 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of MMP9 in human tissues.
Expression of MMP9 in mouse & rat tissues.
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