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宿主来源
Rabbit抗原名称
Transferrin Receptor分子别名
Transferrin receptor protein 1; TR; TfR; TfR1; Trfr; T9; Transferrin receptor 1; p90; CD71; TFRC细胞定位
Secreted, Cell membraneAccession
P02786克隆号
S-4791抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt阳性样本
Daudi, K562, HeLa, HUVEC, 293T, SH-SY5Y, HT-29, LoVo, RAW264.7, mouse brain, mouse lung,纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
ICC ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000-1:2000 Hu, Ms IHC-P 1:1000 Hu, Ms, Rt ICC 1:50 Hu
The Transferrin Receptor (TfR), primarily existing as the homodimeric glycoprotein TfR1 (CD71), is a critical cell surface molecule responsible for regulating cellular iron uptake by binding iron-loaded transferrin with high affinity and internalizing the complex via clathrin-mediated endocytosis, a process essential for delivering iron to the cytoplasm where it supports vital functions such as DNA synthesis, oxygen transport, and mitochondrial respiration; upon iron release in the acidic endosomal environment, the apo-transferrin remains bound to the receptor and is recycled back to the cell surface for dissociation at neutral pH, while the receptor's expression is tightly post-transcriptionally controlled by the Iron Regulatory Protein (IRP) system in response to intracellular iron levels to prevent toxicity or deficiency, making it not only a fundamental component of systemic iron homeostasis but also a significant biomarker for rapidly proliferating cells like erythroid precursors and many cancers, as well as a strategic target for drug delivery systems designed to cross the blood-brain barrier.
免疫印迹
WB result of Transferrin Receptor Recombinant Rabbit mAb
Primary antibody: Transferrin Receptor Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: Daudi whole cell lysate 20 µg
Lane 2: K562 whole cell lysate 20 µg
Lane 3: HeLa whole cell lysate 20 µg
Lane 4: HUVEC whole cell lysate 20 µg
Lane 5: 293T whole cell lysate 20 µg
Lane 6: SH-SY5Y whole cell lysate 20 µg
Lane 7: HT-29 whole cell lysate 20 µg
Lane 8: LoVo whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 85 kDa
Observed MW: 100 kDaWB result of Transferrin Receptor Recombinant Rabbit mAb
Primary antibody: Transferrin Receptor Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: RAW264.7 whole cell lysate 20 µg
Lane 2: mouse brain lysate 20 µg
Lane 3: mouse lung lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 85 kDa
Observed MW: 100 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human cerebral cotex. Anti-Transferrin receptor antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human placenta. Anti-Transferrin receptor antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cervical squamous cell carcinoma Anti-Transferrin receptor antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-Transferrin receptor antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human endometrial cancer. Anti-Transferrin receptor antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-Transferrin receptor antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
ICC shows positive staining in K562 cells. Anti-Transferrin receptor antibody was used at 1/50 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
组织表达图谱
Expression of Transferrin receptor in tumor tissues.
Expression of Transferrin receptor in human tissues.
Expression of Transferrin receptor in mouse & rat tissues.
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