SUV39H1 Recombinant Rabbit mAb (S-3563-3)
Histone-lysine N-methyltransferase SUV39H1,Histone H3-K9 methyltransferase 1 (H3-K9-HMTase 1),Lysine N-methyltransferase 1A,Position-effect variegation 3-9 homolog,Suppressor of variegation 3-9 homolog 1 (Su(var)3-9 homolog 1),KMT1A,SUV39H
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宿主来源
Rabbit抗原名称
SUV39H1分子别名
Histone-lysine N-methyltransferase SUV39H1; Histone H3-K9 methyltransferase 1 (H3-K9-HMTase 1); Lysine N-methyltransferase 1A; Position-effect variegation 3-9 homolog; Suppressor of variegation 3-9 homolog 1 (Su(var)3-9 homolog 1); KMT1A; SUV39H免疫原
Synthetic Peptide细胞定位
Nucleus, Cell membraneAccession
O43463克隆号
S-3563-3抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Mk阳性样本
Ramos, HeLa, HCT 116, NIH/3T3, COS-7纯化方式
Protein A浓度
2 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
ICC ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu, Ms, Mk ICC 1:200 Hu
SUV39H1 (Suppressor of Variegation 3-9 Homolog 1) is a critical histone methyltransferase enzyme that specifically catalyzes the di- and tri-methylation of lysine 9 on histone H3 (H3K9me2/3), a repressive epigenetic mark essential for the formation and maintenance of constitutive heterochromatin, particularly at pericentromeric regions and repetitive DNA sequences. As a member of the SET domain-containing protein family, SUV39H1 plays a pivotal role in preserving genomic stability by silencing transposable elements, preventing chromosomal rearrangements, and ensuring proper chromosome segregation during cell division; its function is tightly regulated through interactions with other chromatin-modifying complexes, including HP1 (Heterochromatin Protein 1), which binds to the H3K9me3 marks created by SUV39H1 to further compact chromatin structure. Dysregulation or mutation of SUV39H1 has been implicated in various pathological conditions, including cancer progression, where altered heterochromatin dynamics can lead to genomic instability and aberrant gene expression, as well as in aging processes, highlighting its significance as a key regulator of epigenetic landscape and cellular identity.
免疫印迹
WB result of SUV39H1 Recombinant Rabbit mAb
Primary antibody: SUV39H1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: Ramos whole cell lysate 20 µg
Lane 2: HeLa whole cell lysate 20 µg
Lane 3: HCT 116 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 48 kDa
Observed MW: 45 kDaWB result of SUV39H1 Recombinant Rabbit mAb
Primary antibody: SUV39H1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 48 kDa
Observed MW: 45 kDaWB result of SUV39H1 Recombinant Rabbit mAb
Primary antibody: SUV39H1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 48 kDa
Observed MW: 45 kDa
免疫细胞化学
ICC shows positive staining in Ramos cells. Anti-SUV39H1 antibody was used at 1/200 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
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