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宿主来源
Rabbit抗原名称
CDK4分子别名
Cyclin-dependent kinase 4; Cell division protein kinase 4; PSK-J3免疫原
Synthetic Peptide细胞定位
Cytoplasm, NucleusAccession
P11802抗体类型
Polyclonal antibody抗体同种型
IgG反应种属 ?
Hu, Ms, Rt, Mk阳性样本
HeLa, MCF7, Jurkat, Neuro-2a, C2C12, CTLL-2, NIH/3T3, MIN6, C6, COS-7纯化方式
Immunogen Affinity浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000-1:2000 Hu, Ms, Rt, Mk IHC-P 1:250 Hu, Ms
Cyclin-dependent kinase 4 (CDK4) is a serine/threonine protein kinase that serves as a critical regulator of the G1-to-S phase transition in the eukaryotic cell cycle by forming an active complex with D-type cyclins (D1, D2, or D3). Upon activation, the CDK4-cyclin D complex phosphorylates the retinoblastoma protein (pRb), causing it to release the E2F family of transcription factors, which subsequently drive the expression of genes essential for DNA replication and cell division. The activity of CDK4 is tightly controlled by various mechanisms, including binding to inhibitors like p16INK4a and post-translational modifications, ensuring that cell proliferation occurs only under appropriate physiological conditions. Due to its pivotal role in promoting uncontrolled cell growth when dysregulated, CDK4 is frequently overexpressed or amplified in numerous cancers, making it a significant therapeutic target for selective small-molecule inhibitors used in treating malignancies such as hormone receptor-positive breast cancer.
免疫印迹
WB result of CDK4 Rabbit pAb
Primary antibody: CDK4 Rabbit pAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: MCF7 whole cell lysate 20 µg
Lane 3: Jurkat whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 33 kDa
Observed MW: 30 kDaWB result of CDK4 Rabbit pAb
Primary antibody: CDK4 Rabbit pAb at 1/1000 dilution
Lane 1: Neuro-2a whole cell lysate 20 µg
Lane 2: C2C12 whole cell lysate 20 µg
Lane 3: CTLL-2 whole cell lysate 20 µg
Lane 4: NIH/3T3 whole cell lysate 20 µg
Lane 5: MIN6 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 33 kDa
Observed MW: 30 kDaWB result of CDK4 Rabbit pAb
Primary antibody: CDK4 Rabbit pAb at 1/1000 dilution
Lane 1: C6 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 33 kDa
Observed MW: 30 kDaWB result of CDK4 Rabbit pAb
Primary antibody: CDK4 Rabbit pAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 33 kDa
Observed MW: 30 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human testis. Anti-CDK4 antibody was used at 1/250 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cervical squamous cell carcinoma. Anti-CDK4 antibody was used at 1/250 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse stomach. Anti-CDK4 antibody was used at 1/250 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of CDK4 in tumor tissues.
Expression of CDK4 in human tissues.
Expression of CDK4 in mouse & rat tissues.
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