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宿主来源
Rabbit抗原名称
CD127分子别名
Interleukin-7 receptor subunit alpha; IL-7 receptor subunit alpha; IL-7R subunit alpha; IL-7R-alpha; IL-7RAAlternative nameCDw127; IL7R细胞定位
Cell membrane, SecretedAccession
P16871克隆号
S-4826抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
稀释度
应用 稀释度 推荐种属 IHC-P 1:250 Hu
CD127, also known as the alpha chain of the interleukin-7 receptor (IL-7Rα), is a type I transmembrane glycoprotein that plays a pivotal role in lymphocyte development, survival, and homeostasis by pairing with the common gamma chain (CD132) to form the functional IL-7 receptor complex. Upon binding to its ligand, IL-7, CD127 triggers downstream signaling cascades, primarily through the JAK1/STAT5 pathway, which are essential for the generation and maintenance of naïve and memory T cells, as well as early B cell development in the bone marrow. Beyond its physiological importance in adaptive immunity, CD127 serves as a critical phenotypic marker in immunology and oncology; it is characteristically expressed on naïve and central memory T cells but downregulated on terminally differentiated effector cells and regulatory T cells (Tregs), making it a valuable tool for distinguishing Treg populations (typically CD4+CD25highCD127low/neg) from activated conventional T cells. Furthermore, aberrant expression or mutations in the gene encoding CD127 (IL7R) have been implicated in various pathologies, including severe combined immunodeficiency (SCID), multiple sclerosis susceptibility, and certain leukemias, highlighting its significance as both a diagnostic marker and a potential therapeutic target for modulating immune responses in autoimmunity, cancer immunotherapy, and immune reconstitution strategies.
免疫组化
IHC shows positive staining in paraffin-embedded human spleen. Anti-CD127 antibody was used at 1/250 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon. Anti-CD127 antibody was used at 1/250 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-CD127 antibody was used at 1/250 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human pancreatic cancer. Anti-CD127 antibody was used at 1/250 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human cerebral cortex. Anti-CD127 antibody was used at 1/250 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of CD127 in tumor tissues.
Expression of CD127 in human tissues.
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