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宿主来源
Rabbit抗原名称
APOL2分子别名
Apolipoprotein L2免疫原
Synthetic Peptide细胞定位
CytoplasmAccession
Q9BQE5克隆号
S-3627-115抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu阳性样本
SK-OV-3, Saos-2, RPMI-8226纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu IHC-P 1:250 Hu
Apolipoprotein L2 (APOL2) is a member of the apolipoprotein L family, primarily synthesized in the liver and secreted into the bloodstream as a component of high-density lipoprotein (HDL) particles, where it plays a significant role in lipid metabolism and reverse cholesterol transport. Structurally, APOL2 shares homology with other APOL proteins, containing an N-terminal signal peptide, a central membrane-associating domain, and a C-terminal SRA-interacting domain, although unlike its paralog APOL1, it does not exhibit trypanolytic activity against Trypanosoma brucei rhodesiense. Beyond its metabolic functions, emerging research suggests that APOL2 may be involved in cellular processes such as autophagy and inflammation, potentially interacting with intracellular membranes to influence vesicle trafficking or serving as a biomarker in certain pathological conditions including kidney disease and cancer, though its precise mechanistic contributions to these non-lipid pathways remain an active area of investigation compared to the more extensively characterized roles of other family members.
免疫印迹
WB result of APOL2 Recombinant Rabbit mAb
Primary antibody: APOL2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: THP-1 whole cell lysate 20 µg
Lane 2: SK-OV-3 whole cell lysate 20 µg
Lane 3: Saos-2 whole cell lysate 20 µg
Low expression control: THP-1 whole cell lysate
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 37 kDa
Observed MW: 37 kDaWB result of APOL2 Recombinant Rabbit mAb
Primary antibody: APOL2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: RPMI-8226 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 37 kDa
Observed MW: 37 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human liver. Anti-APOL2 antibody was used at 1/250 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human placenta. Anti-APOL2 antibody was used at 1/250 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human testis. Anti-APOL2 antibody was used at 1/250 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of APOL2 in human tissues.
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