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宿主来源
Rabbit抗原名称
VDAC3分子别名
Non-selective voltage-gated ion channel VDAC3; VDAC-3; hVDAC3Alternative nameOuter mitochondrial membrane protein porin 3免疫原
Synthetic Peptide细胞定位
MitochondrionAccession
Q9Y277抗体类型
Polyclonal antibody抗体同种型
IgG反应种属 ?
Hu, Ms, Rt阳性样本
HEK293, Jurkat, A431, MCF7, HeLa, HepG2, MEF, NIH/3T3, mouse brain, mouse heart, PC-12, rat brain, rat heart预测反应种属
(反应种属缩写表)BV, Pg, Rb, Or纯化方式
Immunogen Affinity浓度
2 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000-1:2000 Hu, Ms, Rt IHC-P 1:500 Hu, Ms, Rt
VDAC3 (Voltage-Dependent Anion Channel 3) is a crucial isoform of the mitochondrial outer membrane porin family that distinguishes itself from its counterparts, VDAC1 and VDAC2, through unique structural features including an additional cysteine residue which facilitates the formation of intermolecular disulfide bonds and potential oligomerization. Primarily localized in the mitochondria of diverse tissues with particularly high expression in the brain, testes, and skeletal muscle, VDAC3 plays a specialized role in regulating metabolite flux, such as ATP and ADP exchange, while critically contributing to the assembly and stability of the sperm flagellar axoneme, where its dysfunction is directly linked to male infertility due to impaired sperm motility. Beyond its metabolic gating function, VDAC3 is implicated in modulating reactive oxygen species (ROS) levels and interacting with cytoskeletal elements, suggesting a broader involvement in cellular signaling pathways related to oxidative stress response and apoptosis, although its precise gating mechanisms and specific protein-protein interaction networks remain less fully characterized compared to the more ubiquitous VDAC1.
免疫印迹
WB result of VDAC3 Rabbit pAb
Primary antibody: VDAC3 Rabbit pAb at 1/1000 dilution
Lane 1: HEK-293 whole cell lysate 20 µg
Lane 2: Jurkat whole cell lysate 20 µg
Lane 3: A431 whole cell lysate 20 µg
Lane 4: MCF7 whole cell lysate 20 µg
Lane 5: HeLa whole cell lysate 20 µg
Lane 6: HepG2 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 31 kDa
Observed MW: 30 kDaWB result of VDAC3 Rabbit pAb
Primary antibody: VDAC3 Rabbit pAb at 1/1000 dilution
Lane 1: MEF whole cell lysate 20 µg
Lane 2: NIH/3T3 whole cell lysate 20 µg
Lane 3: mouse brain lysate 20 µg
Lane 4: mouse heart lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 31 kDa
Observed MW: 30 kDaWB result of VDAC3 Rabbit pAb
Primary antibody: VDAC3 Rabbit pAb at 1/1000 dilution
Lane 1: PC-12 whole cell lysate 20 µg
Lane 2: rat brain lysate 20 µg
Lane 3: rat heart lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 31 kDa
Observed MW: 30 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human liver. Anti-VDAC3 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human endometrial cancer. Anti-VDAC3 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human ovarian cancer. Anti-VDAC3 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse skeletal muscle. Anti-VDAC3 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat cardiac muscle. Anti-VDAC3 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat stomach. Anti-VDAC3 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of VDAC3 in tumor tissues.
Expression of VDAC3 in human tissues.
Expression of VDAC3 in mouse & rat tissues.
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