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STK39 Recombinant Rabbit mAb (S-3630-15)

STE20/SPS1-related proline-alanine-rich protein kinase,DCHT,Serine/threonine-protein kinase 39,SPAK

价格 ￥600.00 （ 供应商现货 ： 3-5个工作日）
货号 S0B6815
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产品介绍

产品介绍 评论(0)

产品规格

  • 宿主来源

    Rabbit

  • 抗原名称

    STK39

  • 分子别名

    STE20/SPS1-related proline-alanine-rich protein kinase; DCHT; Serine/threonine-protein kinase 39; SPAK

  • 免疫原

    Recombinant Protein

  • 细胞定位

    Cytoplasm, Nucleus

  • Accession

    Q9UEW8

  • 克隆号

    S-3630-15

  • 抗体类型

    Recombinant mAb

  • 抗体同种型

    IgG

  • 反应种属 ?

    Hu, Ms, Rt

  • 阳性样本

    Jurkat, MCF7, MDA-MB-231, SH-SY5Y, Raji, HepG2, mouse brain, rat brain

  • 纯化方式

    Protein A

  • 浓度

    0.5 mg/ml

  • 标记

    Unconjugated

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

  • 应用

    IHC-P ?

    ICC ?

    WB

  • 稀释度

    应用 稀释度 推荐种属
    WB 1:1000 Hu, Ms, Rt
    IHC-P 1:500 Hu, Ms, Rt
    ICC 1:500 Hu
背景介绍

  • STK39 (Serine/Threonine Kinase 39), also known as SIK3 or salt-inducible kinase 3, is a multifunctional enzyme belonging to the AMP-activated protein kinase (AMPK) family within the broader serine/threonine kinase group, playing pivotal roles in regulating cellular metabolism, ion transport, and circadian rhythms. Primarily activated by upstream kinases such as LKB1, STK39 functions as a critical mediator in response to hormonal signals like aldosterone and insulin, where it phosphorylates specific substrates including the sodium-potassium-chloride cotransporter NKCC1 and various transcriptional co-repressors (such as HDAC4 and CRTC2) to modulate electrolyte balance, glucose homeostasis, and lipid metabolism. Beyond its metabolic duties, STK39 is integral to the molecular clock mechanism by influencing the periodicity of circadian gene expression through the regulation of CREB signaling pathways, and emerging research highlights its involvement in pathological conditions, including its potential tumor-suppressive or oncogenic roles depending on tissue context, as well as its contribution to neurodevelopmental disorders and pain sensitivity via neuronal signaling modulation.

  • 免疫印迹

    • WB result of STK39 Recombinant Rabbit mAb
      Primary antibody: STK39 Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: Jurkat whole cell lysate 20 µg
      Lane 2: MCF7 whole cell lysate 20 µg
      Lane 3: MDA-MB-231 whole cell lysate 20 µg
      Lane 4: SH-SY5Y whole cell lysate 20 µg
      Lane 5: Raji whole cell lysate 20 µg
      Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 59 kDa
      Observed MW: 65 kDa
      This blot was developed with high sensitivity substrate

    • WB result of STK39 Recombinant Rabbit mAb
      Primary antibody: STK39 Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: HepG2 whole cell lysate 20 µg
      Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 59 kDa
      Observed MW: 65 kDa

    • WB result of STK39 Recombinant Rabbit mAb
      Primary antibody: STK39 Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: mouse liver lysate 20 µg
      Lane 2: mouse brain lysate 20 µg
      Low expression control: mouse liver lysate
      Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 59 kDa
      Observed MW: 70 kDa
      This blot was developed with high sensitivity substrate

    • WB result of STK39 Recombinant Rabbit mAb
      Primary antibody: STK39 Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: rat liver lysate 20 µg
      Lane 2: rat brain lysate 20 µg
      Low expression control: rat liver lysate
      Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 59 kDa
      Observed MW: 65, 70 kDa
      This blot was developed with high sensitivity substrate

  • 免疫组化

    • IHC shows positive staining in paraffin-embedded human cerebellum. Anti-STK39 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human colon. Anti-STK39 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human colon cancer. Anti-STK39 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human endometrial cancer. Anti-STK39 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human pancreatic cancer. Anti-STK39 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human gastric cancer. Anti-STK39 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-STK39 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti-STK39 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

  • 免疫细胞化学

    • ICC shows positive staining in HepG2 cells. Anti-STK39 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).

  • 组织表达图谱

    • Expression of STK39 in tumor tissues.

    • Expression of STK39 in human tissues.

    • Expression of STK39 in mouse & rat tissues.

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