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宿主来源
Rabbit抗原名称
Phospho-TAZ (ser89)/Phospho-YAP (Ser127)分子别名
WW domain-containing transcription regulator protein 1; Transcriptional coactivator with PDZ-binding motifImported; TAZ s; WWTR1免疫原
Synthetic Peptide细胞定位
Cytoplasm, Nucleus, Cell membraneAccession
Q9GZV5, P46937克隆号
S-1364-129抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Rt阳性样本
PANC-1, HeLa (treated with 100 ng/ml Calyculin A for 30 minutes), C6 (treated with 100 ng/ml Calyculin A for 30 minutes)纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
ICC ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu, Rt ICC 1:500 Hu
Phospho-TAZ (Ser89) and Phospho-YAP (Ser127) are the inactivated forms of the transcriptional coactivators TAZ (also known as WWTR1) and YAP (Yes-associated protein), respectively, following phosphorylation at serine residue 89 and serine residue 127, representing the activation state of the Hippo signaling pathway. These two phosphorylation sites are located within the 14-3-3 protein binding regions of TAZ and YAP, respectively. When the upstream kinase cascade (such as MST1/2 and LATS1/2) is activated, LATS1/2 directly phosphorylates YAP at Ser127 and TAZ at Ser89. The phosphorylated YAP/TAZ then bind to 14-3-3 proteins in the cytoplasm, leading to cytoplasmic sequestration and ubiquitin-mediated degradation, thereby preventing their nuclear translocation and transcriptional regulatory functions. The Hippo signaling pathway regulates the phosphorylation status of TAZ and YAP, participating in the control of organ size, tissue homeostasis, cell proliferation, and apoptosis. In various tumors, reduced phosphorylation levels of TAZ/YAP (i.e., enhanced activation) are commonly observed. These phosphorylation sites are typically detected using specific antibodies via methods such as immunoblotting or immunohistochemistry, serving as key molecular markers for assessing Hippo signaling pathway activity and the nuclear translocation status of TAZ/YAP.
免疫印迹
WB result of Phospho-TAZ (ser89)/Phospho-YAP (Ser127) Recombinant Rabbit mAb
Blocking/Diluting buffer and concentration: 5% NFDM/TBST
Primary antibody: Phospho-TAZ (ser89)/Phospho-YAP (Ser127) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: PANC-1 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 54 kDa
Observed MW: 54 kDa
This blot was developed with high sensitivity substrateWB result of Phospho-TAZ (ser89)/Phospho-YAP (Ser127) Recombinant Rabbit mAb
Blocking/Diluting buffer and concentration: 5% NFDM/TBST
Primary antibody: Phospho-TAZ (ser89)/Phospho-YAP (Ser127) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated HeLa whole cell lysate 20 µg
Lane 2: HeLa treated with 100 ng/ml Calyculin A for 30 minutes whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 54 kDa
Observed MW: 50- 56 kDa
This blot was developed with high sensitivity substrateWB result of Phospho-TAZ (ser89)/Phospho-YAP (Ser127) Recombinant Rabbit mAb
Blocking/Diluting buffer and concentration: 5% NFDM/TBST
Primary antibody: Phospho-TAZ (ser89)/Phospho-YAP (Ser127) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated C6 whole cell lysate 20 µg
Lane 2: C6 treated with 100 ng/ml Calyculin A for 30 minutes whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 54 kDa
Observed MW: 50- 56 kDa
This blot was developed with high sensitivity substrate
免疫细胞化学
ICC analysis of HeLa cells treated with Calyculin A (100ng/ml,30min) (top panel) and untreated HeLa cells (below panel). Anti-Phospho-TAZ (ser89)/ Phospho-YAP (Ser127) antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
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