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Rabbit抗原名称
Phospho-TAK1 (Ser412)分子别名
Mitogen-activated protein kinase kinase kinase 7; Transforming growth factor-beta-activated kinase 1 (TGF-beta-activated kinase 1); TAK1; MAP3K7免疫原
Synthetic Peptide细胞定位
Cell membrane, CytoplasmAccession
O43318克隆号
S-3334-138抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt, Mk阳性样本
HeLa (treated with 100 nM Calyculin A for 10 minutes and 20 ng/ml human IL-1ß for 10 minutes), K562, C2C12, C6, COS-7纯化方式
Protein A浓度
2 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
ICC ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu, Ms, Rt, Mk ICC 1:400 Hu
Phospho-TAK1 (Ser412) is the activated form of transforming growth factor-β-activated kinase 1 (TAK1, also known as MAP3K7) following phosphorylation at serine residue 412, representing the activation state of this serine/threonine kinase signaling pathway. Ser412 is located within the activation loop region of TAK1, and its phosphorylation is critical for TAK1 kinase activity. When cells are stimulated by inflammatory cytokines (such as IL-1β, TNF-α), TGF-β, or various stress signals, TAK1 is activated through ubiquitination-dependent mechanisms. Phosphorylation at Ser412 promotes the interaction of TAK1 with its cofactors TAB1/TAB2/TAB3, subsequently activating downstream NF-κB and MAPK (including JNK and p38) signaling pathways, thereby regulating diverse biological processes such as inflammatory responses, apoptosis, autophagy, and fibrosis. Studies have shown that aberrant phosphorylation at Ser412 is closely associated with sustained activation of TAK1 in autoimmune diseases, inflammatory bowel disease, liver fibrosis, and various cancers.
免疫印迹
WB result of Phospho-TAK1 (Ser412) Recombinant Rabbit mAb
Blocking/Diluting buffer and concentration: 5% NFDM/TBST
Primary antibody incubation conditions: overnight at 4°C
Primary antibody: Phospho-TAK1 (Ser412) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated HeLa whole cell lysate 20 µg
Lane 2: HeLa treated with 100 nM Calyculin A for 10 minutes and 20 ng/ml human IL-1ß for 10 minutes whole cell lysate 20 µg
Lane 3: K562 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 67 kDa
Observed MW: 70 kDaWB result of Phospho-TAK1 (Ser412) Recombinant Rabbit mAb
Blocking/Diluting buffer and concentration: 5% NFDM/TBST
Primary antibody incubation conditions: overnight at 4°C
Primary antibody: Phospho-TAK1 (Ser412) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: C2C12 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 67 kDa
Observed MW: 70 kDaWB result of Phospho-TAK1 (Ser412) Recombinant Rabbit mAb
Blocking/Diluting buffer and concentration: 5% NFDM/TBST
Primary antibody incubation conditions: overnight at 4°C
Primary antibody: Phospho-TAK1 (Ser412) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: C6 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 67 kDa
Observed MW: 70 kDaWB result of Phospho-TAK1 (Ser412) Recombinant Rabbit mAb
Blocking/Diluting buffer and concentration: 5% NFDM/TBST
Primary antibody incubation conditions: overnight at 4°C
Primary antibody: Phospho-TAK1 (Ser412) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 67 kDa
Observed MW: 70 kDa
免疫细胞化学
ICC analysis of HeLa cells treated with Calyculin A(100nM,10min) and human IL-1ß (20ng/ml 10min) (top panel) and untreated HeLa cells (below panel). Anti-Phospho-TAK1 (Ser412) antibody was used at 1/400 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
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