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Rabbit抗原名称
Phospho-IRF-3 (Ser396)分子别名
Interferon regulatory factor 3; IRF3细胞定位
NucleusAccession
Q14653克隆号
S-3598抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu阳性样本
HT-29 (transfected with 2.5 μg/ml Poly (I:C) for 6 hours)纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
ICC ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu ICC 1:500 Hu
Phospho-IRF3 (Ser396) refers to the activated form of Interferon Regulatory Factor 3 (IRF-3), a critical transcription factor in the innate immune system, specifically phosphorylated at serine residue 396 within its C-terminal regulatory domain. This post-translational modification is primarily catalyzed by the kinases TBK1 and IKKε following the detection of viral pathogens by pattern recognition receptors such as TLR3, TLR4, or cytosolic sensors like RIG-I and MDA5. Upon phosphorylation at Ser396, IRF-3 undergoes a conformational change that disrupts its autoinhibitory state, promoting homodimerization and subsequent translocation from the cytoplasm into the nucleus. Once inside the nucleus, the phospho-IRF-3 dimer binds to interferon-stimulated response elements (ISREs) in the promoter regions of target genes, driving the robust transcription of type I interferons (particularly IFN-β) and other antiviral cytokines, thereby establishing an essential antiviral state to limit viral replication and spread.
免疫印迹
WB result of Phospho-IRF-3 (Ser396) Recombinant Rabbit mAb
Blocking/Diluting buffer and concentration: 5% NFDM/TBST
Primary antibody incubation conditions: overnight at 4°C
Primary antibody: Phospho-IRF-3 (Ser396) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untransfected HT-29 whole cell lysate 20 µg
Lane 2: HT-29 transfected with 2.5 μg/ml Poly (I:C) for 6 hours whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 47 kDa
Observed MW: 56 kDa
This blot was developed with high sensitivity substrate
免疫细胞化学
ICC analysis of HT-29 cells treated with Poly (I:C) (2.5 μg/ml, 6 hr) (top panel) and untreated HT-29 cells (below panel). Anti-Phospho-IRF3 (Ser396) antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
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