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NKR-P1C Recombinant Rabbit mAb (S-4706)

Killer cell lectin-like receptor subfamily B member 1C,CD161 antigen-like family member C,Lymphocyte antigen 55c (Ly-55c),NK1.1,NKR-P1.9,Natural killer cell surface protein P1-40 (NKR-P1 40),CD161c,Ly55c,Nkrp1c,Klrb1c

价格 ￥600.00 （ 供应商现货 ： 3-5个工作日）
货号 S0B6810
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产品介绍

产品介绍 评论(0)

产品规格

  • 宿主来源

    Rabbit

  • 抗原名称

    NKR-P1C

  • 分子别名

    Killer cell lectin-like receptor subfamily B member 1C; CD161 antigen-like family member C; Lymphocyte antigen 55c (Ly-55c); NK1.1; NKR-P1.9; Natural killer cell surface protein P1-40 (NKR-P1 40); CD161c; Ly55c; Nkrp1c; Klrb1c

  • Accession

    P27814

  • 克隆号

    S-4706

  • 抗体类型

    Recombinant mAb

  • 抗体同种型

    IgG

  • 反应种属 ?

    Ms

  • 阳性样本

    mouse splenocytes

  • 纯化方式

    Protein A

  • 浓度

    0.5 mg/ml

  • 标记

    Unconjugated

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

  • 应用

    IHC-P ?

    FCM

    ICC ?

  • 稀释度

    应用 稀释度 推荐种属
    IHC-P 1:100 Ms
    ICC 1:500 Ms
    FCM 1:100 Ms
背景介绍

  • NKR-P1C, also known as NK1.1 or CD161c, is a type II transmembrane protein primarily expressed on the surface of natural killer (NK) cells in mice, belonging to the C-type lectin-like receptor family. As an activating receptor, it plays a central role: upon cross-linking by its ligand (such as the virus-encoded decoy molecule m12), it recruits the tyrosine kinase p56lck, initiating downstream signaling cascades that induce calcium influx, thereby enhancing NK cell cytotoxicity and stimulating the production of interferon-gamma (IFN-γ). Notably, due to its encoding by a specific gene, the expression of NKR-P1C is strain-specific; it is commonly found in mice such as C57BL/6 but is naturally absent in strains like BALB/c, making it a key phenotypic marker for identifying NK cells in these specific mouse strains.

  • 流式分析

    • Flow cytometric analysis of C57BL/6 mouse splenocytes labeled Mouse NKR-P1C antibody at 1/100 dilution (0.5 μg) / (right panel) compared with a Rabbit IgG Isotype Control / (left panel). Goat Anti- Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody. Then cells were stained with CD49b – APC antibody separately. Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.

  • 免疫组化

    • IHC shows positive staining in paraffin-embedded mouse spleen. Anti-NKR-P1C antibody was used at 1/100 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded mouse colon. Anti-NKR-P1C antibody was used at 1/100 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

  • 免疫细胞化学

    • ICC shows positive staining in mouse splenocytes. Anti-NKR-P1C antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).

  • 组织表达图谱

    • Expression of NKR-P1C in mouse & rat tissues.

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