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宿主来源
Rabbit抗原名称
MAGE-A4分子别名
Melanoma-associated antigen 4; Cancer/testis antigen 1.4 (CT1.4); MAGE-4 antigen; MAGE-41 antigen; MAGE-X2 antigen; MAGE4; MAGEA4免疫原
Synthetic Peptide细胞定位
NucleusAccession
P43358克隆号
S-3868-66抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu IHC-P 1:2000 Hu
MAGE-A4 (Melanoma-Associated Antigen A4) is a cancer-testis antigen belonging to the MAGE-A family, which is normally expressed exclusively in immune-privileged sites such as the testis and placenta but is aberrantly reactivated in a wide variety of malignant tumors including synovial sarcoma, lung squamous cell carcinoma, head and neck cancers, and melanoma. As an intracellular protein processed and presented on the cell surface by Major Histocompatibility Complex (MHC) class I molecules, MAGE-A4 serves as a highly specific tumor-associated antigen that is largely absent from normal somatic tissues, making it an attractive target for immunotherapeutic strategies such as T-cell receptor (TCR)-engineered T cells, peptide vaccines, and antibody-drug conjugates. Its expression is often correlated with tumor progression and poor prognosis in certain cancers, and because of its restricted normal tissue distribution, therapies targeting MAGE-A4 aim to maximize tumor killing while minimizing off-target toxicity, although careful monitoring is required due to potential low-level expression in some normal tissues or the risk of cross-reactivity with other MAGE family members.
免疫印迹
WB result of MAGE-A4 Recombinant Rabbit mAb
Primary antibody: MAGE-A4 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: MCF-7 whole cell lysate 20 µg
Lane 2: U-2 OS whole cell lysate 20 µg
Lane 3: A431 whole cell lysate 20 µg
Negative control: MCF-7 whole cell lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 39 kDa
Observed MW: 40 kDa
免疫组化
Negative control: IHC shows negative staining in paraffin-embedded human colon. Anti- MAGE-A4 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human lung. Anti- MAGE-A4 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human testis. Anti-MAGE-A4 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung squamous cell carcinoma Anti-MAGE-A4 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of MAGE-A4 in tumor tissues.
Expression of MAGE-A4 in human tissues.
Expression of MAGE-A4 in lung cancer tissues.
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