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GluN3B Recombinant Rabbit mAb (S-2320-3)

Glutamate receptor ionotropic,NMDA 3B,GRIN3B

价格 600.00 供应商现货 : 3-5个工作日
货号 S0B6805
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产品介绍 评论(0)

产品规格
  • 宿主来源

    Rabbit
  • 抗原名称

    GluN3B
  • 分子别名

    Glutamate receptor ionotropic, NMDA 3B; GRIN3B
  • 免疫原

    Recombinant Protein
  • 细胞定位

    Cell membrane
  • Accession

    O60391
  • 克隆号

    S-2320-3
  • 抗体类型

    Recombinant mAb
  • 抗体同种型

    IgG
  • 反应种属 ?

    Hu, Ms
  • 阳性样本

    CHO transfected with GluN3B-Myc-His fusion protein
  • 纯化方式

    Protein A
  • 浓度

    0.5 mg/ml
  • 标记

    Unconjugated
  • 性状

    Liquid
  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

  • 应用

    ICC ?

    WB

  • 稀释度

    应用 稀释度 推荐种属
    WB 1:500-1:1000 Ms
    ICC 1:500 Ms
背景介绍
  • GluN3B, encoded by the GRIN3B gene, is a unique and atypical subunit of the N-methyl-D-aspartate receptor (NMDAR) that fundamentally alters the receptor's functional properties when co-assembled with obligatory GluN1 subunits and conventional GluN2 subunits. Unlike other NMDAR subunits, GluN3B contains a distinct ligand-binding domain that renders the resulting heterotrimeric or heterotetrameric receptors insensitive to the co-agonist glycine and significantly reduces their calcium permeability and single-channel conductance, effectively acting as a dominant-negative modulator that dampens excitatory synaptic transmission. Primarily expressed in specific brain regions such as the thalamus, striatum, and during early postnatal development in the cortex, GluN3B plays a critical role in refining neural circuits by limiting excessive NMDAR activity, thereby influencing processes like synaptic plasticity, motor coordination, and sensory gating; furthermore, dysregulation or mutations in GRIN3B have been implicated in various neurological disorders, including schizophrenia, Huntington's disease, and rare forms of epilepsy, highlighting its importance as a potential therapeutic target for modulating glutamatergic signaling without completely abolishing NMDAR function.

  • 免疫印迹

    • WB result of GluN3B Recombinant Rabbit mAb
      Primary antibody: GluN3B Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: CHO transfected with empty vector whole cell lysate 20 µg
      Lane 2: CHO transfected with GluN3B-Myc-His fusion protein whole cell lysate 20 µg
      Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 113 kDa
      Observed MW: 115 kDa

  • 免疫细胞化学

    • ICC shows positive staining in GluN3B-Myc-His fusion protein transfected CHO cells (top panel) and negative staining in vector-transfected CHO cells (below panel). Anti- GluN3B antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Mouse IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).

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