CDS2 Recombinant Rabbit mAb (S-3670-56)
Phosphatidate cytidylyltransferase 2,CDP-DAG synthase 2,CDP-DG synthase 2,CDP-diacylglycerol synthase 2 (CDS 2),CDP-diglyceride pyrophosphorylase 2,CDP-diglyceride synthase 2,CTP:phosphatidate cytidylyltransferase 2
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宿主来源
Rabbit抗原名称
CDS2分子别名
Phosphatidate cytidylyltransferase 2; CDP-DAG synthase 2; CDP-DG synthase 2; CDP-diacylglycerol synthase 2 (CDS 2); CDP-diglyceride pyrophosphorylase 2; CDP-diglyceride synthase 2; CTP:phosphatidate cytidylyltransferase 2免疫原
Synthetic Peptide细胞定位
Endoplasmic reticulumAccession
O95674克隆号
S-3670-56抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt, Mk阳性样本
HepG2, PC-3, HeLa, NIH/3T3, PC-12, COS-7纯化方式
Protein A浓度
2 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu, Ms, Rt, Mk IHC-P 1:250 Hu, Ms
CDP-diacylglycerol synthase 2 (CDS2), also known as CDP-DAG synthase 2, is a critical membrane-bound enzyme located primarily in the endoplasmic reticulum that catalyzes the conversion of phosphatidic acid and cytidine triphosphate (CTP) into cytidine diphosphate-diacylglycerol (CDP-DAG), serving as the committed and rate-limiting step in the de novo biosynthesis of major phospholipids such as phosphatidylinositol, phosphatidylglycerol, and cardiolipin. As one of two isoforms in mammals (the other being CDS1), CDS2 plays an indispensable role in maintaining cellular membrane integrity, facilitating intracellular signaling pathways dependent on phosphoinositides, and regulating lipid homeostasis, with its expression and activity being tightly linked to metabolic processes where dysregulation has been associated with various pathologies including lipodystrophy, insulin resistance, and altered susceptibility to viral infections due to its involvement in modifying host membrane composition required for viral replication.
免疫印迹
WB result of CDS2 Recombinant Rabbit mAb
Primary antibody: CDS2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: PC-12 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 51 kDa
Observed MW: 52 kDaWB result of CDS2 Recombinant Rabbit mAb
Primary antibody: CDS2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 51 kDa
Observed MW: 52 kDaWB result of CDS2 Recombinant Rabbit mAb
Primary antibody: CDS2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HepG2 whole cell lysate 20 µg
Lane 2: PC-3 whole cell lysate 20 µg
Lane 3: HeLa whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 51 kDa
Observed MW: 52 kDaWB result of CDS2 Recombinant Rabbit mAb
Primary antibody: CDS2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 51 kDa
Observed MW: 52 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human liver. Anti-CDS2 antibody was used at 1/250 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-CDS2 antibody was used at 1/250 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-CDS2 antibody was used at 1/250 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of CDS2 in tumor tissues.
Expression of CDS2 in human tissues.
Expression of CDS2 in mouse & rat tissues.
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