CDS1 Recombinant Rabbit mAb (S-3671-30)
Phosphatidate cytidylyltransferase 1,CDP-DAG synthase 1,CDP-DG synthase 1,CDP-diacylglycerol synthase 1 (CDS 1),CDP-diglyceride pyrophosphorylase 1,CDP-diglyceride synthase 1,CTP:phosphatidate cytidylyltransferase 1,CDS
分享产品介绍 评论(0)
宿主来源
Rabbit抗原名称
CDS1分子别名
Phosphatidate cytidylyltransferase 1; CDP-DAG synthase 1; CDP-DG synthase 1; CDP-diacylglycerol synthase 1 (CDS 1); CDP-diglyceride pyrophosphorylase 1; CDP-diglyceride synthase 1; CTP:phosphatidate cytidylyltransferase 1; CDS免疫原
Synthetic Peptide细胞定位
Endoplasmic reticulumAccession
Q92903克隆号
S-3671-30抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt阳性样本
SK-BR-3, A431, PC-3, RAW264.7纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
ICC ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000-1:2000 Hu, Ms IHC-P 1:1000 Hu, Ms, Rt ICC 1:500 Hu
CDP-diacylglycerol synthase 1 (CDS1), also known as CDP-DAG synthase 1, is a critical integral membrane enzyme primarily located in the endoplasmic reticulum that catalyzes the conversion of phosphatidic acid (PA) and cytidine triphosphate (CTP) into cytidine diphosphate-diacylglycerol (CDP-DAG), serving as the committed and rate-limiting step in the de novo biosynthesis of major phospholipids such as phosphatidylinositol (PI), phosphatidylglycerol (PG), and cardiolipin. As a central hub in lipid metabolism, CDS1 regulates the flux of PA toward phospholipid production rather than triglyceride synthesis or signaling pathways, thereby maintaining cellular membrane integrity, facilitating signal transduction cascades like the PI3K/Akt pathway, and supporting mitochondrial function through cardiolipin generation; its activity is tightly regulated by feedback inhibition from downstream products and transcriptional mechanisms, and dysregulation or mutations in the CDS1 gene have been implicated in severe metabolic disorders, including congenital lipodystrophy, insulin resistance, and altered susceptibility to viral infections due to compromised membrane architecture.
免疫印迹
WB result of CDS1 Recombinant Rabbit mAb
Primary antibody: CDS1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: unboiled A549 whole cell lysate 20 µg
Lane 2: unboiled SK-BR-3 whole cell lysate 20 µg
Low expression control: unboiled A549 whole cell lysate
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 53 kDa
Observed MW: 53 kDaWB result of CDS1 Recombinant Rabbit mAb
Primary antibody: CDS1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: unboiled A431 whole cell lysate 20 µg
Lane 2: unboiled PC-3 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 53 kDa
Observed MW: 53 kDaWB result of CDS1 Recombinant Rabbit mAb
Primary antibody: CDS1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: unboiled RAW264.7 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 53 kDa
Observed MW: 53 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-CDS1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon. Anti-CDS1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-CDS1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human liver cancer. Anti-CDS1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-CDS1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat stomach. Anti-CDS1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
ICC shows positive staining in SK-BR-3 cells (top panel) and negative staining in A549 cells (below panel). Anti-CDS1 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
组织表达图谱
Expression of CDS1 in tumor tissues.
Expression of CDS1 in human tissues.
Expression of CDS1 in mouse & rat tissues.
评论(0)