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宿主来源
Rabbit抗原名称
SIDT2分子别名
SID1 transmembrane family member 2细胞定位
Cell membrane, LysosomeAccession
Q8NBJ9克隆号
S-3763-76抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Mk阳性样本
A204, SK-BR-3, Caco-2, HeLa, COS-7纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
ICC ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu, Mk IHC-P 1:250 Hu ICC 1:100 Hu
SIDT2 (Systemic RNA Interference Defective Transmembrane Protein 2) is a highly conserved transmembrane protein primarily localized to the lysosomal membrane in mammals, where it functions as a critical regulator of nucleic acid transport and lysosomal homeostasis. Originally identified through homology with the C. elegans SID-1 protein involved in systemic RNA interference, mammalian SIDT2 has evolved distinct roles centered on facilitating the uptake of extracellular double-stranded RNA (dsRNA) and mediating the degradation of intracellular RNA within lysosomes, thereby influencing gene silencing pathways and immune responses. Beyond its involvement in RNA metabolism, SIDT2 plays a significant part in autophagic flux by promoting the fusion of autophagosomes with lysosomes and assisting in the clearance of protein aggregates, which is particularly vital for neuronal health; consequently, dysregulation or mutations in the SIDT2 gene have been linked to neurodegenerative conditions, metabolic disorders, and altered susceptibility to viral infections, highlighting its multifaceted importance in maintaining cellular stability and managing nucleic acid trafficking across biological barriers.
免疫印迹
WB result of SIDT2 Recombinant Rabbit mAb
Primary antibody: SIDT2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: unboiled A204 whole cell lysate 20 µg
Lane 2: unboiled SK-BR-3 whole cell lysate 20 µg
Lane 3: unboiled Caco-2 whole cell lysate 20 µg
Lane 4: unboiled HeLa whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 94 kDa
Observed MW: 105 kDaWB result of SIDT2 Recombinant Rabbit mAb
Primary antibody: SIDT2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: unboiled COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 94 kDa
Observed MW: 105 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-SIDT2 antibody was used at 1/250 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human breast cancer. Anti-SIDT2 antibody was used at 1/250 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human gastric cancer. Anti-SIDT2 antibody was used at 1/250 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
ICC shows positive staining in Caco2 cells. Anti-SIDT2 antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
组织表达图谱
Expression of SIDT2 in tumor tissues.
Expression of SIDT2 in human tissues.
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