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宿主来源
Rabbit抗原名称
Phospho-Myosin Light Chain 2 (Ser19)分子别名
Myosin regulatory light polypeptide 9, Myosin regulatory light chain 2, smooth muscle isoform, Myosin regulatory light chain 9, Myl9, Myrl2免疫原
Synthetic Peptide细胞定位
Cytoplasm, CytoskeletonAccession
Q9CQ19克隆号
S-3335-18抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Ms阳性样本
C2C12, C2C12 (treated with 50 µM ML-7 for 15 minutes)纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
WB
稀释度
应用 稀释度 推荐种属 Dot Blot 1:1000 WB 1:1000-1:2000 Ms
Phospho-Myosin Light Chain 2 (Ser19) refers to the specific post-translational modification of the regulatory myosin light chain (MLC2, also known as MLC20 in smooth muscle or MLC2a/2b in striated muscle) where a phosphate group is added to the serine residue at position 19. This phosphorylation event is primarily catalyzed by Myosin Light Chain Kinase (MLCK) in response to increased intracellular calcium levels, although Rho-associated kinase (ROCK) can also contribute, particularly in smooth muscle tone regulation. The addition of the phosphate group at Ser19 induces a conformational change in the myosin head, enabling it to interact with actin filaments and initiate ATP-dependent cross-bridge cycling, which is the fundamental mechanism driving muscle contraction and non-muscle cell motility, such as cytokinesis and cell migration. Consequently, detection of Phospho-MLC2 (Ser19) is widely used as a key biomarker for assessing cellular contractility and cytoskeletal dynamics in various physiological and pathological contexts.
免疫印迹
WB result of Phospho-Myosin Light Chain 2 (Ser19) Recombinant Rabbit mAb
Blocking/Diluting buffer and concentration: 5% NFDM/TBST
Primary antibody: Phospho-Myosin Light Chain 2 (Ser19) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated C2C12 whole cell lysate 20 µg
Lane 2: C2C12 treated with 50 µM ML-7 for 15 minutes whole cell lysate 20 µg
Lane 3: C2C12 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 20 kDa
Observed MW: 22 kDa
斑点杂交
Dot blot result of Phospho-Myosin Light Chain 2 (Ser19) Recombinant Rabbit mAb
Lane 1: Myosin Light Chain 2 (Ser19) phospho peptide
Lane 2: Myosin Light Chain 2 phospho peptide
Lane 3: Myosin Light Chain 2 (Ser19) unmodified peptide
Primary antibody: Phospho-Myosin Light Chain 2 (Ser19) Recombinant Rabbit mAb at 1/1000 dilution
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
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