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宿主来源
Rabbit抗原名称
Histone H3 (acetyl K23)分子别名
H3K23ac免疫原
Synthetic Peptide细胞定位
NucleusAccession
P68431克隆号
S-3321-202抗体类型
Recombinant mAb抗体同种型
IgG翻译后修饰类型
乙酰化反应种属 ?
Hu, Ms, Rt阳性样本
MCF7 (treated with 50 mM sodium butyrate for 24 hours and 500 ng/ml TSA for 4 hours), NIH/3T3 (treated with 500 ng/ml TSA for 4 hours), C6 (treated with 500 ng/ml TSA for 4 hours), HeLa (treated with 500ng/ml TSA for 4 hours)纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
ICC ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000-1:2000 Hu, Ms, Rt IHC-P 1:2500 Hu, Ms, Rt ICC 1:500 Hu
Histone H3 lysine 23 acetylation (H3K23ac) is a crucial epigenetic modification characterized by the addition of an acetyl group to the lysine residue at position 23 on the N-terminal tail of histone H3, a process catalyzed by specific histone acetyltransferases (such as p300/CBP) and reversible by deacetylases. Distinct from many other acetylation marks (e.g., H3K9ac, H3K27ac) that primarily enrich at active promoters or enhancers, H3K23ac exhibits a unique genomic distribution; it is widely present in euchromatin and notably enriched within gene bodies, particularly in exons. This specific localization suggests its function extends beyond transcriptional initiation to include roles in regulating transcription elongation, facilitating splice site recognition, and maintaining an open chromatin structure to prevent the erroneous spread of heterochromatin. Furthermore, H3K23ac plays a pivotal role in DNA damage response and specific developmental stages, while its dysregulation has been linked to various cancers and neurodevelopmental disorders, establishing it as an emerging and critical regulatory marker in contemporary epigenetic research.
免疫印迹
WB result of Histone H3 (acetyl K23) Recombinant Rabbit mAb
Primary antibody: Histone H3 (acetyl K23) Recombinant Rabbit mAb at 1/5000 dilution
Lane 1: untreated MCF7 whole cell lysate 20 µg
Lane 2: MCF7 treated with 50 mM sodium butyrate for 24 hours and 500 ng/ml TSA for 4 hours whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 15 kDa
Observed MW: 17 kDaWB result of Histone H3 (acetyl K23) Recombinant Rabbit mAb
Primary antibody: Histone H3 (acetyl K23) Recombinant Rabbit mAb at 1/5000 dilution
Lane 1: untreated NIH/3T3 whole cell lysate 20 µg
Lane 2: NIH/3T3 treated with 500 ng/ml TSA for 4 hours whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 15 kDa
Observed MW: 17 kDaWB result of Histone H3 (acetyl K23) Recombinant Rabbit mAb
Primary antibody: Histone H3 (acetyl K23) Recombinant Rabbit mAb at 1/5000 dilution
Lane 1: untreated C6 whole cell lysate 20 µg
Lane 2: C6 treated with 500 ng/ml TSA for 4 hours whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 15 kDa
Observed MW: 17 kDa
斑点杂交
Dot blot result of Histone H3 (acetyl K23) Recombinant Rabbit mAb
Lane 1: Histone H3 K23ac modified peptide
Lane 2: Histone H3 K23 unmodified peptide
Primary antibody: Histone H3 (acetyl K23) Recombinant Rabbit mAb at 1/1000 dilution
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
免疫组化
IHC shows positive staining in paraffin-embedded human colon. Anti-Histone H3 (acetyl K23) antibody was used at 1/2500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cervical squamous cell carcinoma. Anti-Histone H3 (acetyl K23) antibody was used at 1/2500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human thyroid cancer. Anti-Histone H3 (acetyl K23) antibody was used at 1/2500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse colon. Anti-Histone H3 (acetyl K23) antibody was used at 1/2500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat lung. Anti-Histone H3 (acetyl K23) antibody was used at 1/2500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
ICC analysis of HeLa cells treated with TSA (500ng/ml, 4h) (top panel) and untreated HeLa cells (below panel). Anti-Histone H3 (acetyl K23) antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
组织表达图谱
Expression of Histone H3 (acetyl K23) in tumor tissues.
Expression of Histone H3 (acetyl K23) in human tissues.
Expression of Histone H3 (acetyl K23) in mouse & rat tissues.
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