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GPR40 Recombinant Rabbit mAb (S-3817-50)

Free fatty acid receptor 1,FFAR1

价格 ￥600.00 （ 供应商现货 ： 3-5个工作日）
货号 S0B6793
规格
数量
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产品介绍

产品介绍 评论(0)

产品规格

  • 宿主来源

    Rabbit

  • 抗原名称

    GPR40

  • 分子别名

    Free fatty acid receptor 1; FFAR1

  • 免疫原

    Synthetic Peptide

  • 细胞定位

    Cell membrane

  • Accession

    O14842

  • 克隆号

    S-3817-50

  • 抗体类型

    Recombinant mAb

  • 抗体同种型

    IgG

  • 反应种属 ?

    Hu, Ms, Rt, Mk

  • 阳性样本

    HepG2, MCF7, MIN6, mouse pancreas, rat pancreas, COS-7

  • 纯化方式

    Protein A

  • 浓度

    0.5 mg/ml

  • 标记

    Unconjugated

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

  • 应用

    WB

  • 稀释度

    应用 稀释度 推荐种属
    WB 1:1000 Hu, Ms, Rt, Mk
背景介绍

  • GPR40, also known as Free Fatty Acid Receptor 1 (FFAR1), is a G protein-coupled receptor primarily expressed in pancreatic beta cells and enteroendocrine cells that plays a pivotal role in metabolic regulation by sensing circulating medium- and long-chain free fatty acids. Upon activation by these dietary lipids, GPR40 triggers an intracellular signaling cascade involving Gq/11 proteins, which leads to increased phospholipase C activity, elevated intracellular calcium levels, and the subsequent potentiation of glucose-stimulated insulin secretion (GSIS) without directly inducing insulin release in the absence of glucose. This unique mechanism makes GPR40 a highly attractive therapeutic target for type 2 diabetes, as its agonists can enhance insulin output in a glucose-dependent manner, thereby minimizing the risk of hypoglycemia, while also stimulating the release of incretin hormones like GLP-1 from intestinal L-cells to further improve glycemic control and potentially preserve beta-cell function.

  • 免疫印迹

    • WB result of GPR40 Recombinant Rabbit mAb
      Primary antibody: GPR40 Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: HepG2 whole cell lysate 20 µg
      Lane 2: MCF7 whole cell lysate 20 µg
      Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 31 kDa
      Observed MW: 25 kDa

    • WB result of GPR40 Recombinant Rabbit mAb
      Primary antibody: GPR40 Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: MIN6 whole cell lysate 20 µg
      Lane 2: mouse pancreas lysate 20 µg
      Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 31 kDa
      Observed MW: 25 kDa

    • WB result of GPR40 Recombinant Rabbit mAb
      Primary antibody: GPR40 Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: rat pancreas lysate 20 µg
      Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 31 kDa
      Observed MW: 25 kDa

    • WB result of GPR40 Recombinant Rabbit mAb
      Primary antibody: GPR40 Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: COS-7 whole cell lysate 20 µg
      Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 31 kDa
      Observed MW: 28 kDa

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