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宿主来源
Mouse抗原名称
BRCA1分子别名
Breast cancer type 1 susceptibility protein; RING finger protein 53; RING-type E3 ubiquitin transferase BRCA1; RNF53细胞定位
Nucleus, CytoplasmAccession
P38398克隆号
S-4707抗体类型
Mouse mAb抗体同种型
IgG1,k反应种属 ?
Hu纯化方式
Protein G浓度
2 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
ICC ?
稀释度
应用 稀释度 推荐种属 IHC-P 1:1000 Hu ICC 1:2000 Hu
BRCA1 (Breast Cancer gene 1) is a critical tumor suppressor protein encoded by the BRCA1 gene in humans, playing an indispensable role in maintaining genomic stability primarily through its involvement in the high-fidelity repair of double-strand DNA breaks via homologous recombination. Functioning as a scaffold within a large multiprotein complex, BRCA1 interacts with key partners such as BARD1, PALB2, and RAD51 to coordinate cell cycle checkpoints, particularly at the G2/M transition, ensuring that cells do not divide with damaged DNA, while also participating in transcriptional regulation and ubiquitination processes; consequently, inherited loss-of-function mutations in the BRCA1 gene severely compromise these DNA repair mechanisms, leading to an accumulation of genetic errors and a significantly elevated lifetime risk of developing hereditary breast and ovarian cancers, which has made the protein a central focus for both genetic screening protocols and the development of targeted therapies like PARP inhibitors that exploit synthetic lethality in BRCA1-deficient tumors.
免疫组化
IHC shows positive staining in paraffin-embedded human breast. Anti-BRCA1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human breast cancer (case 1). Anti-BRCA1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human breast cancer (case 2). Anti-BRCA1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows negative staining in paraffin-embedded human breast cancer (case 3). Anti-BRCA1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
ICC shows positive staining in MCF7 cells. Anti-BRCA1 antibody was used at 1/2000 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Mouse IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
组织表达图谱
Expression of BRCA1 in tumor tissues.
Expression of BRCA1 in human tissues.
Expression of BRCA1 in human breast tumor tissues.
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