NF-κB2 p100/p52 Recombinant Rabbit mAb (S-2502-208)
Nuclear factor NF-kappa-B p100 subunit,DNA-binding factor KBF2,H2TF1,Lymphocyte translocation chromosome 10 protein,Nuclear factor of kappa light polypeptide gene enhancer in B-cells 2,Oncogene Lyt-10 (Lyt10),LYT10,NFKB2
分享产品介绍 评论(0)
宿主来源
Rabbit抗原名称
NF-κB2 p100/p52分子别名
Nuclear factor NF-kappa-B p100 subunit; DNA-binding factor KBF2; H2TF1; Lymphocyte translocation chromosome 10 protein; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 2; Oncogene Lyt-10 (Lyt10); LYT10; NFKB2免疫原
Recombinant Protein细胞定位
Cytoplasm, NucleusAccession
Q00653克隆号
S-2502-208抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt, Mk阳性样本
HDLM-2, Karpas-299, NIH/3T3, L-929, C2C12, J774A.1, C6, Rat spleen, COS-7纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
ICC ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu, Ms, Rt, Mk IHC-P 1:200 Hu ICC 1:100 Ms
NF-κB2 p100 is a 100 kDa cytoplasmic precursor protein encoded by the nfkb2 gene that functions both as an IκB-like inhibitor of NF-κB—sequestering RelB and other subunits in the cytoplasm—and as the obligate source of the mature NF-κB2 p52 subunit generated via stimulus-induced, phosphorylation- and ubiquitin-dependent partial proteasomal processing triggered by the alternative NF-κB pathway kinases NIK and IKKα, thereby enabling p52-RelB dimers to translocate to the nucleus and drive transcriptional programs essential for lymphoid organogenesis, B-cell maturation and adaptive immunity.
免疫印迹
WB result of NF-κB2 p100/p52 Recombinant Rabbit mAb
Primary antibody: NF-κB2 p100/p52 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HDLM-2 whole cell lysate 20 µg
Lane 2: Karpas-299 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 49, 97 kDa
Observed MW: 52, 120 kDaWB result of NF-κB2 p100/p52 Recombinant Rabbit mAb
Primary antibody: NF-κB2 p100/p52 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: L-929 whole cell lysate 20 µg
Lane 3: C2C12 whole cell lysate 20 µg
Lane 4: J774A.1 whole cell lysate 20 µg
Lane 5: mouse spleen lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 49, 97 kDa
Observed MW: 52, 120 kDaWB result of NF-κB2 p100/p52 Recombinant Rabbit mAb
Primary antibody: NF-κB2 p100/p52 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: C6 whole cell lysate 20 µg
Lane 2: rat spleen lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 49, 97 kDa
Observed MW: 52, 120 kDaWB result of NF-κB2 p100/p52 Recombinant Rabbit mAb
Primary antibody: NF-κB2 p100/p52 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 49, 97 kDa
Observed MW: 52, 120 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human testis. Anti- NF-κB2 p100/p52 antibody was used at 1/200 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human tonsil. Anti- NF-κB2 p100/p52 antibody was used at 1/200 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human endometrial cancer. Anti- NF-κB2 p100/p52 antibody was used at 1/200 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human ovarian cancer. Anti- NF-κB2 p100/p52 antibody was used at 1/200 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
ICC shows positive staining in NIH/3T3 cells. Anti- NF-κB2 p100/p52 antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
组织表达图谱
Expression of NF-κB2 p100/p52 in tumor tissues.
Expression of NF-κB2 p100/p52 in human tissues.
评论(0)