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宿主来源
Rabbit抗原名称
FDX1分子别名
Adrenodoxin, mitochondrial; Adrenal ferredoxin; Ferredoxin-1; Hepatoredoxin; ADX免疫原
Recombinant Protein细胞定位
MitochondrionAccession
P10109克隆号
S-3587-58抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt阳性样本
A549, HepG2, PC-3, Caco-2, mouse kidney, mouse testis, rat kidney, rat testis, COS-7预测反应种属
(反应种属缩写表)Bv, Pg, Sh, Ck纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
IF ?
稀释度
应用 稀释度 推荐种属 WB 1:1000-1:2000 Hu, Ms, Rt, Mk IHC-P 1:200 Hu, Ms, Rt IF 1:500 Ms
FDX1 (Ferredoxin 1) is a crucial mitochondrial iron-sulfur cluster protein that serves as an essential electron donor for the biosynthesis of iron-sulfur clusters and steroid hormones, functioning primarily by transferring electrons from NADPH via ferredoxin reductase (FDXR) to various target enzymes such as ferredoxin-dependent sulfite reductase and cytochrome P450 family members involved in processes like heme synthesis, vitamin D activation, and cortisol production; notably, recent groundbreaking research has identified FDX1 as the central upstream regulator of cuproptosis, a distinct form of regulated cell death triggered by excessive copper accumulation, wherein FDX1 directly reduces Cu2+ to the more toxic Cu+ state and facilitates the lipoylation of tricarboxylic acid cycle proteins, leading to their aggregation and subsequent proteotoxic stress that ultimately causes cell demise, thereby establishing FDX1 not only as a vital metabolic hub but also as a potential therapeutic target for cancer treatment and copper-related disorders.
免疫印迹
WB result of FDX1 Recombinant Rabbit mAb
Primary antibody: FDX1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: A549 whole cell lysate 20 µg
Lane 2: HepG2 whole cell lysate 20 µg
Lane 3: PC-3 whole cell lysate 20 µg
Lane 4: Caco-2 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 19 kDa
Observed MW: 14 kDaWB result of FDX1 Recombinant Rabbit mAb
Primary antibody: FDX1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse kidney lysate 20 µg
Lane 2: mouse testis lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 19 kDa
Observed MW: 14 kDaWB result of FDX1 Recombinant Rabbit mAb
Primary antibody: FDX1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat kidney lysate 20 µg
Lane 2: rat testis lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 19 kDa
Observed MW: 14 kDaWB result of FDX1 Recombinant Rabbit mAb
Primary antibody: FDX1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 19 kDa
Observed MW: 14 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human adrenal gland. Anti-FDX1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-FDX1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human kidney. Anti-FDX1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human testis. Anti-FDX1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human hepatocellular carcinoma. Anti-FDX1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫荧光
IF shows positive staining in paraffin-embedded mouse adrenal gland. Anti-FDX1 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
组织表达图谱
Expression of FDX1 in tumor tissues.
Expression of FDX1 in human tissues.
Expression of FDX1 in mouse & rat tissues.
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