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Rabbit抗原名称
DAZL分子别名
Deleted in azoospermia-like; DAZ-like autosomal; Deleted in azoospermia-like 1; Dazl1; Dazla; Dazl免疫原
Recombinant Protein细胞定位
CytoplasmAccession
Q64368克隆号
S-3113-35抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt纯化方式
Protein A浓度
0.2 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
稀释度
应用 稀释度 推荐种属 IHC-P 1:2000 Hu, Ms, Rt
DAZL (Deleted in Azoospermia-Like) is a highly conserved, germ-cell-specific RNA-binding protein that orchestrates key post-transcriptional events across vertebrate gametogenesis. It carries an N-terminal RNA-recognition motif and a C-terminal DAZ repeat that jointly bind U-rich sequences in the 3′ UTRs of target mRNAs, enabling DAZL to recruit poly(A)-binding proteins (PABPs) and stimulate cap-dependent translation initiation independently of the poly(A) tail length. Expressed from primordial germ cell (PGC) specification through meiotic progression in both sexes, DAZL licenses these cells to respond to retinoic acid, thereby promoting meiotic entry, synaptonemal complex formation via SYCP3, and spindle assembly during oocyte maturation. In vivo, loss of DAZL causes complete germ-cell depletion and infertility, whereas haploinsufficiency yields abnormal sperm and ovarian insufficiency, underscoring its role as a master regulator of germline survival and differentiation.
免疫组化
IHC shows positive staining in paraffin-embedded human testis. Anti-DAZL antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human kidney. Anti-DAZL antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse testis. Anti-DAZL antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded mouse kidney. Anti-DAZL antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat testis. Anti-DAZL antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded rat kidney. Anti-DAZL antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of DAZL in human tissues.
Expression of DAZL in mouse & rat tissues.
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