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Rabbit抗原名称
BMI-1分子别名
Polycomb complex protein BMI-1; Polycomb group RING finger protein 4; RING finger protein 51; PCGF4; RNF51; BMI1;Bmi1免疫原
Synthetic Peptide细胞定位
Cytoplasm, NucleusAccession
P35226克隆号
S-3313-71抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu阳性样本
A549, HeLa, U-2 OS, Molt-4预测反应种属
(反应种属缩写表)Bv, Ct纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
ICC ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu IHC-P 1:200 Hu ICC 1:500 Hu
BMI-1 (B-cell-specific Moloney murine leukemia virus integration site 1) is a critical polycomb group protein that functions as an essential component of the Polycomb Repressive Complex 1 (PRC1), playing a pivotal role in epigenetic gene silencing by catalyzing the monoubiquitination of histone H2A at lysine 119, thereby repressing key target genes such as the INK4a/ARF locus (which encodes tumor suppressors p16INK4a and p14ARF) to regulate cell cycle progression, cellular senescence, and stem cell self-renewal; beyond its normal physiological functions in development and hematopoiesis, BMI-1 is frequently overexpressed in various malignancies, where it drives tumorigenesis, promotes cancer stem cell maintenance, confers resistance to chemotherapy and radiation, and serves as a significant prognostic marker and potential therapeutic target across numerous cancer types including breast, lung, and colorectal cancers.
免疫印迹
WB result of BMI-1 Recombinant Rabbit mAb
Primary antibody: BMI-1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: A549 whole cell lysate 20 µg
Lane 2: HeLa whole cell lysate 20 µg
Lane 3: U-2 OS whole cell lysate 20 µg
Lane 4: Molt-4 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 37 kDa
Observed MW: 40 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human tonsil. Anti-BMI-1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon. Anti-BMI-1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-BMI-1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung adenocarcinoma. Anti-BMI-1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
ICC shows positive staining in HeLa cells. Anti-BMI-1 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
组织表达图谱
Expression of BMI-1 in tumor tissues.
Expression of BMI-1 in human tissues.
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