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Rabbit抗原名称
PSMB5分子别名
Proteasome subunit beta type-5; Macropain epsilon chain; Multicatalytic endopeptidase complex epsilon chain; Proteasome chain 6; Proteasome epsilon chain; Proteasome subunit MB1; LMPX; MB1; X免疫原
Synthetic Peptide细胞定位
Nucleus, CytoplasmAccession
P28074抗体类型
Polyclonal antibody抗体同种型
IgG反应种属 ?
Hu, Ms, Rt, Mk阳性样本
K562, RPMI-8226, MCF7, T47D, Neuro-2a, mouse liver, COS-7纯化方式
Immunogen Affinity浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:2000 Hu, Ms, Mk IHC-P 1:200 Hu, Ms, Rt
PSMB5, also known as proteasome subunit beta type-5 or the X subunit of the 20S core particle, is a critical catalytic component of the 26S proteasome complex responsible for intracellular protein degradation via the ubiquitin-proteasome pathway. As one of the three distinct beta subunits possessing proteolytic activity within the 20S core, PSMB5 specifically exhibits chymotrypsin-like activity, cleaving peptide bonds adjacent to hydrophobic amino acid residues, which is essential for maintaining cellular homeostasis by removing damaged, misfolded, or regulatory proteins. This subunit plays a pivotal role in antigen processing for MHC class I presentation, thereby influencing immune surveillance, and serves as the primary molecular target for bortezomib and other proteasome inhibitor drugs used clinically to treat multiple myeloma and mantle cell lymphoma; furthermore, under inflammatory conditions induced by interferon-gamma, PSMB5 can be replaced by its inducible homolog LMP7 (PSMB8) to form the immunoproteasome, which alters cleavage specificity to optimize the generation of antigenic peptides for immune recognition.
免疫印迹
WB result of PSMB5 Rabbit pAb
Primary antibody: PSMB5 Rabbit pAb at 1/1000 dilution
Lane 1: K562 whole cell lysate 20 µg
Lane 2: RPMI-8226 whole cell lysate 20 µg
Lane 3: MCF7 whole cell lysate 20 µg
Lane 4: T-47D whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 28 kDa
Observed MW: 23 kDaWB result of PSMB5 Rabbit pAb
Primary antibody: PSMB5 Rabbit pAb at 1/1000 dilution
Lane 1: Neuro-2a whole cell lysate 20 µg
Lane 2: mouse liver lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 28 kDa
Observed MW: 23 kDaWB result of PSMB5 Rabbit pAb
Primary antibody: PSMB5 Rabbit pAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 28 kDa
Observed MW: 23 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-PSMB5 antibody was used at 1/200 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human kidney. Anti-PSMB5 antibody was used at 1/200 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human breast cancer. Anti-PSMB5 antibody was used at 1/200 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human hepatocellular carcinoma. Anti-PSMB5 antibody was used at 1/200 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse cerebral cortx. Anti-PSMB5 antibody was used at 1/200 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti-PSMB5 antibody was used at 1/200 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of PSMB5 in tumor tissues.
Expression of PSMB5 in human tissues.
Expression of PSMB5 in mouse &rat tissues.
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