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宿主来源
Rabbit抗原名称
SERPINB3分子别名
Serpin B3; Protein T4-A; Squamous cell carcinoma antigen 1 (SCCA-1); SCCA; SCCA1细胞定位
Secreted, CytoplasmAccession
P29508克隆号
S-4587抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu阳性样本
A431, BxPC-3纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
IF ?
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu IHC-P 1:2000 Hu IF 1:500 Hu
SERPINB3, also known as Squamous Cell Carcinoma Antigen 1 (SCCA1), is a member of the clade B serpin family that functions primarily as an intracellular inhibitor of cysteine and serine proteases, playing a critical role in regulating apoptosis and protecting cells from protease-induced damage. Normally expressed in stratified squamous epithelia such as the skin, cervix, and lung, this protein helps maintain tissue integrity by inhibiting enzymes like cathepsins and papain-like proteases; however, its expression is frequently and significantly upregulated in various malignancies, particularly squamous cell carcinomas of the head and neck, lung, esophagus, and cervix, where it contributes to tumor progression by conferring resistance to chemotherapy and radiation through the suppression of granzyme B-mediated cytotoxicity and the inhibition of Fas-mediated apoptotic pathways. Due to its strong association with advanced disease stages, poor prognosis, and therapeutic resistance, SERPINB3 serves as a valuable clinical biomarker for monitoring tumor burden and recurrence, while also emerging as a potential therapeutic target aimed at restoring apoptotic sensitivity in resistant cancer cells.
免疫印迹
WB result of SERPINB3 Recombinant Rabbit mAb
Primary antibody incubation conditions: overnight at 4°C
Primary antibody: SERPINB3 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: IMR-32 whole cell lysate 20 µg
Lane 2: HL-60 whole cell lysate 20 µg
Lane 3: HepG2 whole cell lysate 20 µg
Lane 4: A431 whole cell lysate 20 µg
Lane 5: BxPC-3 whole cell lysate 20 µg
Negative control: IMR-32 whole cell lysate; HL-60 whole cell lysate; HepG2 whole cell lysate
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 45 kDa
Observed MW: 45 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human esophagus. Anti-SERPINB3 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human bladder cancer. Anti-SERPINB3 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cervical squamous cell carcainoma. Anti-SERPINB3 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human pancreatic cancer. Anti-SERPINB3 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫荧光
IF shows positive staining in paraffin-embedded human esophagus. Anti- SERPINB3 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
组织表达图谱
Expression of SERPINB3 in tumor tissues.
Expression of SERPINB3 in human tissues.
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