Versican Recombinant Rabbit mAb (S-3770-43)
Versican core protein,Chondroitin sulfate proteoglycan core protein 2 (Chondroitin sulfate proteoglycan 2),Glial hyaluronate-binding protein (GHAP),Large fibroblast proteoglycan,PG-M,CSPG2,VCAN
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宿主来源
Rabbit抗原名称
Versican分子别名
Versican core protein; Chondroitin sulfate proteoglycan core protein 2 (Chondroitin sulfate proteoglycan 2); Glial hyaluronate-binding protein (GHAP); Large fibroblast proteoglycan; PG-M; CSPG2; VCAN免疫原
Synthetic Peptide细胞定位
SecretedAccession
P13611克隆号
S-3770-43抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt阳性样本
mouse hippocampus, rat hippocampus预测反应种属
(反应种属缩写表)PiMq纯化方式
Protein A浓度
2 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:500-1:1000 Ms, Rt IHC-P 1:2000 Hu, Ms, Rt
Versican is a large extracellular matrix proteoglycan primarily secreted by mesenchymal cells and belonging to the hyaluronan-binding lectican family; its core structure comprises an N-terminal G1 domain (responsible for stable binding to hyaluronan and link proteins), a central region containing glycosaminoglycan (GAG) attachment domains (GAG-β and/or GAG-α) that are covalently linked to chondroitin sulfate chains to confer significant hydration capacity, turgor pressure, and elastic buffering to tissues, and a C-terminal G3 domain that mediates interactions with other matrix components such as fibulins, selectins, and growth factors. This protein is critical during embryonic development for heart formation, neural crest cell migration, and limb morphogenesis, while in adult tissues it plays a widespread role in vascular homeostasis, wound healing, and the regulation of inflammatory responses; moreover, its aberrant overexpression is closely associated with the remodeling of the tumor microenvironment, where it promotes cancer cell proliferation, migration, invasion, and immune evasion, establishing Versican as a key biomarker for the progression and poor prognosis of various malignant tumors.
免疫印迹
WB result of Versican Recombinant Rabbit mAb
Primary antibody: Versican Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: MDA-MB-231 whole cell lysate 20 µg
Lane 2: HeLa whole cell lysate 20 µg
Lane 3: mouse hippocampus lysate 20 µg
Negative control: MDA-MB-231 whole cell lysate; HeLa whole cell lysate
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 373 kDa
Observed MW: 300-400 kDa
This blot was developed with high sensitivity substrateWB result of Versican Recombinant Rabbit mAb
Primary antibody: Versican Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat hippocampus lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 373 kDa
Observed MW: 300-400 kDa
This blot was developed with high sensitivity substrate
免疫组化
IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-Versican antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon. Anti-Versican antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human testis. Anti-Versican antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cervical squamous cell carcinoma. Anti-Versican antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human endometrial cancer. Anti-Versican antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human pancreatic cancer. Anti-Versican antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-Versican antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti-Versican antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat stomach. Anti-Versican antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of Versican in tumor tissues.
Expression of Versican in human tissue.
Expression of Versican in mouse &rat tissues.
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