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Rabbit抗原名称
TOP2A分子别名
DNA topoisomerase 2-alpha; TOP2免疫原
Synthetic Peptide细胞定位
Cytoplasm, NucleusAccession
P11388克隆号
S-513-107抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt阳性样本
SK-BR-3, A549, A431, HeLa, Neuro-2a, mouse testis纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
ICC ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu, Ms IHC-P 1:2000 Hu, Ms, Rt ICC 1:500 Hu
TOP2A (DNA topoisomerase IIα) is a crucial nuclear enzyme highly expressed during the S and G2/M phases of the cell cycle, responsible for resolving topological challenges arising from DNA replication and chromosome segregation; it functions by creating transient double-strand breaks in DNA, allowing another DNA duplex to pass through the gap before resealing the ends, thereby effectively relieving supercoiling tension, decatenating interlinked DNA rings, and ensuring the accurate separation of sister chromatids during anaphase. As a hallmark of cell proliferation, TOP2A exhibits high activity in rapidly dividing tissues, and its dysfunction can lead to genomic instability and chromosomal aberrations; furthermore, it serves as a primary target for important anticancer drugs such as anthracyclines (e.g., doxorubicin) and epipodophyllotoxins (e.g., etoposide), which exert their cytotoxic effects by stabilizing the TOP2A-DNA covalent complex to induce lethal double-strand breaks, ultimately inhibiting tumor cell growth.
免疫印迹
WB result of TOP2A Recombinant Rabbit mAb
Primary antibody: TOP2A Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: WI-38 whole cell lysate 20 µg
Lane 2: SK-BR-3 whole cell lysate 20 µg
Lane 3: A549 whole cell lysate 20 µg
Lane 4: A431 whole cell lysate 20 µg
Lane 5: HeLa whole cell lysate 20 µg
Low expression control: WI-38 whole cell lysate
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 174 kDa
Observed MW: 190 kDaWB result of TOP2A Recombinant Rabbit mAb
Primary antibody: TOP2A Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: Neuro-2a whole cell lysate 20 µg
Lane 2: mouse testis lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 174 kDa
Observed MW: 190 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human colon. Anti-TOP2A antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human testis. Anti-TOP2A antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human tonsil. Anti-TOP2A antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cervical squamous cell carcinoma. Anti-TOP2A antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human endometrial cancer. Anti-TOP2A antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human gastric cancer. Anti-TOP2A antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse colon. Anti-TOP2A antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat testis. Anti-TOP2A antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
ICC shows positive staining in HeLa cells. Anti-TOP2A antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
组织表达图谱
Expression of TOP2A in tumor tissues.
Expression of TOP2A in human tissues.
Expression of TOP2A in mouse & rat tissues.
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