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Rabbit抗原名称
PTRF分子别名
Caveolae-associated protein 1; Cavin-1; Polymerase I and transcript release factor; CAVIN1免疫原
Recombinant Protein细胞定位
Cell membrane, Cytoplasm, Nucleus, MitochondrionAccession
Q6NZI2克隆号
S-3271-13抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu阳性样本
SK-OV-3, PACN-1纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000-1:2000 Hu IHC-P 1:10000 Hu
免疫印迹
WB result of PTRF Recombinant Rabbit mAb
Primary antibody: PTRF Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: Ramos whole cell lysate 20 µg
Lane 2: SK-OV-3 whole cell lysate 20 µg
Lane 3: PANC-1 whole cell lysate 20 µg
Negative control: Ramos whole cell lysate
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 43 kDa
Observed MW: 55 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human kidney. Anti-PTRF antibody was used at 1/10000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human liver. Anti-PTRF antibody was used at 1/10000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human testis. Anti-PTRF antibody was used at 1/10000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human ovarian cancer. Anti-PTRF antibody was used at 1/10000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human prostatic cancer. Anti-PTRF antibody was used at 1/10000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of PTRF in tumor tissues.
Expression of PTRF in human tissues.







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