Phospho-SMAD2/3 (Thr8) Recombinant Rabbit mAb (S-3372-136)
SMAD family member 2,hSMAD2,JV18-1,Mad-related protein 2 (hMAD-2),Mothers against decapentaplegic homolog 2 (MAD homolog 2,Mothers against DPP homolog 2),MADH2,MADR2,SMAD family member 3,hSMAD3,JV15-2,Mothers against decapentaplegic homolog 3 (MAD homolog 3,Mad3,Mothers against DPP homolog 3,hMAD-3),MADH3
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宿主来源
Rabbit抗原名称
Phospho-SMAD2/3 (Thr8)分子别名
SMAD family member 2; hSMAD2; JV18-1; Mad-related protein 2 (hMAD-2); Mothers against decapentaplegic homolog 2 (MAD homolog 2; Mothers against DPP homolog 2); MADH2; MADR2; SMAD family member 3; hSMAD3; JV15-2; Mothers against decapentaplegic homolog 3 (MAD homolog 3; Mad3; Mothers against DPP homolog 3; hMAD-3); MADH3免疫原
Synthetic Peptide细胞定位
Cytoplasm, NucleusAccession
Q15796, P84022克隆号
S-3372-136抗体类型
Recombinant mAb抗体同种型
IgG翻译后修饰类型
磷酸化反应种属 ?
Hu, Ms, Rt阳性样本
293T, RAW264.7, PC-12纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
WB
稀释度
应用 稀释度 推荐种属 Dot Blot 1:1000 WB 1:1000 Hu, Ms, Rt
Phospho-SMAD2/3 (Thr8) refers to the phosphorylation of SMAD2 and SMAD3 proteins at threonine 8, a site distinct from their canonical C-terminal SXS motif phosphorylation by TGF-β receptors. This alternative phosphorylation is typically mediated by MAP kinases (ERK, JNK, p38) or CDKs in response to various stimuli including growth factors, stress, and cell cycle signals. It modulates SMAD transcriptional activity, stability, and subcellular localization, serving as a regulatory crossroad between TGF-β signaling and other pathways. Aberrant Thr8 phosphorylation has been implicated in cancer progression and fibrotic diseases, influencing cell proliferation, migration, and epithelial-mesenchymal transition.
免疫印迹
WB result of Phospho-SMAD2/3 (Thr8) Recombinant Rabbit mAb
Blocking/Diluting buffer and concentration: 5% NFDM/TBST
Primary antibody: Phospho-SMAD2/3 (Thr8) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: 293T whole cell lysate 20 µg
Lane 2: 293T whole cell lysate 20 µg (phosphatase treated membrane)
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 52 kDa
Observed MW: 52, 56 kDaWB result of Phospho-SMAD2/3 (Thr8) Recombinant Rabbit mAb
Blocking/Diluting buffer and concentration: 5% NFDM/TBST
Primary antibody: Phospho-SMAD2/3 (Thr8) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: RAW264.7 whole cell lysate 20 µg
Lane 2: RAW264.7 whole cell lysate 20 µg (phosphatase treated membrane)
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 52 kDa
Observed MW: 56 kDaWB result of Phospho-SMAD2/3 (Thr8) Recombinant Rabbit mAb
Blocking/Diluting buffer and concentration: 5% NFDM/TBST
Primary antibody: Phospho-SMAD2/3 (Thr8) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: PC-12 whole cell lysate 20 µg
Lane 2: PC-12 whole cell lysate 20 µg (phosphatase treated membrane)
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 52 kDa
Observed MW: 56 kDa
斑点杂交
Dot blot result of Phospho-SMAD2/3 (Thr8) Recombinant Rabbit mAb
Lane 1: SMAD2/3 (Thr8) phospho peptide
Lane 2: SMAD2/3 (Thr8) unmodified peptide
Primary antibody: Phospho-SMAD2/3 (Thr8) Recombinant Rabbit mAb at 1/1000 dilution
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
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