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Phospho-Erk5 (Thr218/Tyr220) Recombinant Rabbit mAb (S-3538-42)

Mitogen-activated protein kinase 7,MAP kinase 7,MAPK 7,Big MAP kinase 1 (BMK-1),Extracellular signal-regulated kinase 5 (ERK-5),BMK1,ERK5,PRKM7,MAPK7

价格 ￥600.00 （ 供应商现货 ： 3-5个工作日）
货号 S0B6749
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产品介绍

产品介绍 评论(0)

产品规格

  • 宿主来源

    Rabbit

  • 抗原名称

    Phospho-Erk5 (Thr218/Tyr220)

  • 分子别名

    Mitogen-activated protein kinase 7; MAP kinase 7; MAPK 7; Big MAP kinase 1 (BMK-1); Extracellular signal-regulated kinase 5 (ERK-5); BMK1; ERK5; PRKM7; MAPK7

  • 免疫原

    Synthetic Peptide

  • 细胞定位

    Nucleus, Cytoplasm

  • Accession

    Q13164

  • 克隆号

    S-3538-42

  • 抗体类型

    Recombinant mAb

  • 抗体同种型

    IgG

  • 翻译后修饰类型

    磷酸化

  • 反应种属 ?

    Hu, Ms

  • 阳性样本

    HeLa (starved overnight, then treated with 50 ng/ml hEGF for 10 minutes), NIH/3T3 (starved overnight, then treated with 100 ng/ml PDGF for 5 minutes)

  • 纯化方式

    Protein A

  • 浓度

    0.5 mg/ml

  • 标记

    Unconjugated

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

  • 应用

    WB

  • 稀释度

    应用 稀释度 推荐种属
    WB 1:1000 Hu, Ms
背景介绍

  • Phospho-Erk5 (Thr218/Tyr220) is a phosphorylated form of the extracellular signal-regulated kinase 5 (Erk5), also known as big MAP kinase 1 (BMK1). It is activated through dual phosphorylation at threonine 218 and tyrosine 220 within its TEY activation motif by upstream MAP kinase kinases MEK5. This phosphorylation event is essential for Erk5 catalytic activity and its subsequent nuclear translocation, where it regulates gene expression involved in cell proliferation, survival, and differentiation. Phospho-Erk5 serves as a specific biomarker for Erk5 pathway activation in various cellular processes and disease states, including cancer progression and cardiovascular development.

  • 免疫印迹

    • WB result of Phospho-Erk5 (Thr218/Tyr220) Recombinant Rabbit mAb
      Blocking/Diluting buffer and concentration: 5% NFDM/TBST
      Primary antibody incubation conditions: overnight at 4°C
      Primary antibody: Phospho-Erk5 (Thr218/Tyr220) Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: untreated HeLa whole cell lysate 20 µg
      Lane 2: HeLa starved overnight, then treated with 50 ng/ml hEGF for 10 minutes whole cell lysate 20 µg
      Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 88 kDa
      Observed MW: 190 kDa

    • WB result of Phospho-Erk5 (Thr218/Tyr220) Recombinant Rabbit mAb
      Blocking/Diluting buffer and concentration: 5% NFDM/TBST
      Primary antibody incubation conditions: overnight at 4°C
      Primary antibody: Phospho-Erk5 (Thr218/Tyr220) Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: untreated NIH/3T3 whole cell lysate 20 µg
      Lane 2: NIH/3T3 starved overnight, then treated with 100 ng/ml PDGF for 5 minutes whole cell lysate 20 µg
      Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 88 kDa
      Observed MW: 190 kDa

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