MID51 Recombinant Rabbit mAb (S-3570-28)
Mitochondrial dynamics protein MIEF1,Mitochondrial dynamics protein of 51 kDa,Mitochondrial elongation factor 1,Smith-Magenis syndrome chromosomal region candidate gene 7 protein-like (SMCR7-like protein),SMCR7L,MIEF1
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宿主来源
Rabbit抗原名称
MID51分子别名
Mitochondrial dynamics protein MIEF1; Mitochondrial dynamics protein of 51 kDa; Mitochondrial elongation factor 1; Smith-Magenis syndrome chromosomal region candidate gene 7 protein-like (SMCR7-like protein); SMCR7L; MIEF1免疫原
Synthetic Peptide细胞定位
MitochondrionAccession
Q9NQG6克隆号
S-3570-28抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt, Mk阳性样本
293T, A549, HT-1080, HeLa, NIH/3T3, mouse testis, PC-12, rat testis, COS-7纯化方式
Protein A浓度
2 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu, Ms, Rt, Mk
MID51 (Mitochondrial Dynamics Protein of 51 kDa), also known as MIEF1 (Mitochondrial Elongation Factor 1) or SMCR7L, is a unique outer mitochondrial membrane (OMM) protein that plays a pivotal and somewhat paradoxical role in regulating mitochondrial dynamics by primarily promoting mitochondrial fusion and inhibiting fission. Unlike the canonical fission mediator Drp1 (Dynamin-related protein 1), which MID51 physically recruits to the mitochondrial surface, MID51 acts as a dominant-negative regulator that sequesters Drp1 into inactive spiral structures, thereby preventing Drp1 from executing membrane constriction and division. Structurally, MID51 contains an N-terminal transmembrane domain anchoring it to the OMM and a C-terminal nucleotidyltransferase-like domain that, despite retaining the structural fold, has lost catalytic activity and instead functions as a protein-protein interaction hub essential for Drp1 binding. Beyond its role in maintaining elongated mitochondrial networks crucial for cellular energy homeostasis and stress resistance, MID51 is implicated in various physiological and pathological processes, including cardiomyocyte function, neuronal development, and viral immune evasion (e.g., by hepatitis C virus), where its dysregulation can lead to fragmented mitochondria, impaired bioenergetics, and increased susceptibility to apoptosis.
免疫印迹
WB result of MID51 Recombinant Rabbit mAb
Primary antibody: MID51 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: 293T whole cell lysate 20 µg
Lane 2: A549 whole cell lysate 20 µg
Lane 3: HT-1080 whole cell lysate 20 µg
Lane 4: HeLa whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 51 kDa
Observed MW: 51 kDa
This blot was developed with high sensitivity substrateWB result of MID51 Recombinant Rabbit mAb
Primary antibody: MID51 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: mouse testis lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 51 kDa
Observed MW: 51 kDa
This blot was developed with high sensitivity substrateWB result of MID51 Recombinant Rabbit mAb
Primary antibody: MID51 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: PC-12 whole cell lysate 20 µg
Lane 2: rat testis lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 51 kDa
Observed MW: 51 kDa
This blot was developed with high sensitivity substrateWB result of MID51 Recombinant Rabbit mAb
Primary antibody: MID51 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 51 kDa
Observed MW: 51 kDa
This blot was developed with high sensitivity substrate
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