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产品中心 蛋白翻译后修饰(PTM)抗体 位点特异性修饰抗体

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Histone H4 (Lactyl K8) Recombinant Rabbit mAb (S-3243-36)

H4K8la

价格 ￥600.00 （ 供应商现货 ： 3-5个工作日）
货号 S0B6745
规格
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产品介绍

产品介绍 评论(0)

产品规格

  • 宿主来源

    Rabbit

  • 抗原名称

    Histone H4 (Lactyl K8)

  • 分子别名

    H4K8la

  • 免疫原

    Synthetic Peptide

  • 细胞定位

    Nucleus

  • Accession

    P62805

  • 克隆号

    S-3243-36

  • 抗体类型

    Recombinant mAb

  • 抗体同种型

    IgG

  • 翻译后修饰类型

    乳酸化

  • 反应种属 ?

    Hu, Ms, Rt

  • 阳性样本

    HeLa treated with 100 mM Lactate sodium for 24 hours, NIH/3T3 treated with 100 mM Lactate sodium for 24 hours

  • 纯化方式

    Protein A

  • 浓度

    0.5 mg/ml

  • 标记

    Unconjugated

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

  • 应用

    IHC-P ?

    ICC ?

    WB

  • 稀释度

    应用 稀释度 推荐种属
    WB 1:1000-1:2000 Hu, Ms
    IHC-P 1:500 Hu, Ms, Rt
    ICC 1:500 Hu
背景介绍

  • Histone H4 (Lactyl K8), abbreviated as H4K8la, is a novel epigenetic modification involving the covalent attachment of a lactyl group to the 8th lysine residue of the histone H4 tail, serving as a direct molecular link between cellular glycolysis and gene transcriptional regulation. Discovered as part of the broader "histone lactylation" landscape, this mark is dynamically induced by high intracellular lactate levels, often resulting from hypoxia, intense exercise, or the Warburg effect in cancer cells. Functionally, H4K8la is strongly associated with the activation of specific gene programs; for instance, in macrophages, it accumulates during the late phase of polarization to promote the expression of reparative (M2-like) genes, thereby facilitating tissue repair and resolving inflammation, while in tumor microenvironments, it can drive the expression of oncogenes or factors supporting immune evasion. Mechanistically, the presence of the lactyl group at K8 alters chromatin structure and recruits specific "reader" proteins to enhance transcriptional elongation or initiation, distinguishing its regulatory role from the more constitutive H4K8 acetylation and highlighting its importance as a metabolic checkpoint in immunity, cancer progression, and developmental biology.

  • 免疫印迹

    • WB result of Histone H4 (Lactyl K8) Recombinant Rabbit mAb
      Primary antibody: Histone H4 (Lactyl K8) Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: untreated HeLa whole cell lysate 20 µg
      Lane 2: HeLa treated with 100 mM Lactate sodium for 24 hours whole cell lysate 20 µg
      Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 11 kDa
      Observed MW: 14 kDa

    • WB result of Histone H4 (Lactyl K8) Recombinant Rabbit mAb
      Primary antibody: Histone H4 (Lactyl K8) Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: untreated NIH/3T3 whole cell lysate 20 µg
      Lane 2: NIH/3T3 treated with 100 mM Lactate sodium for 24 hours whole cell lysate 20 µg
      Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 11 kDa
      Observed MW: 14 kDa

  • 斑点杂交

    • Dot blot result of Histone H4 (Lactyl K8) Recombinant Rabbit mAb
      Lane 1: Histone H4 (Lactyl K8) modified peptide
      Lane 2: Histone H4 (Lactyl K8) unmodified peptide control
      Primary antibody: Histone H4 (Lactyl K8) Recombinant Rabbit mAb at 1/1000 dilution
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution

  • 免疫组化

    • IHC shows positive staining in paraffin-embedded human colon. Anti-Histone H4 (Lactyl K8) antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human stomach. Anti-Histone H4 (Lactyl K8) antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human colon cancer. Anti-Histone H4 (Lactyl K8) antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human stomach cancer. Anti-Histone H4 (Lactyl K8) antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human endometrial cancer. Anti-Histone H4 (Lactyl K8) antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded mouse colon. Anti-Histone H4 (Lactyl K8) antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded mouse stomach. Anti-Histone H4 (Lactyl K8) antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded rat colon. Anti-Histone H4 (Lactyl K8) antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded rat stomach. Anti-Histone H4 (Lactyl K8) antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

  • 免疫细胞化学

    • ICC analysis of HeLa cells treated with Lactate sodium (100 mM, 24 hours) (top panel) and untreated HeLa cells (below panel). Anti-Histone H4 (Lactyl K8) antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).

  • 组织表达图谱

    • Expression of Histone H4 (Lactyl K8) in tumor tissues.

    • Expression of Histone H4 (Lactyl K8) in human tissues.

    • Expression of Histone H4 (Lactyl K8) in mouse & rat tissues.

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