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产品中心 蛋白翻译后修饰(PTM)抗体 位点特异性修饰抗体

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Histone H4 (Lactyl K5) Recombinant Rabbit mAb (S-3220-73)

H4K5la

价格 ￥600.00 （ 供应商现货 ： 3-5个工作日）
货号 S0B6744
规格
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产品介绍

产品介绍 评论(0)

产品规格

  • 宿主来源

    Rabbit

  • 抗原名称

    Histone H4 (Lactyl K5)

  • 分子别名

    H4K5la

  • 免疫原

    Synthetic Peptide

  • 细胞定位

    Nucleus

  • Accession

    P62805

  • 克隆号

    S-3220-73

  • 抗体类型

    Recombinant mAb

  • 抗体同种型

    IgG

  • 翻译后修饰类型

    乳酸化

  • 反应种属 ?

    Hu, Ms

  • 阳性样本

    HeLa treated with 100 mM Lactate sodium for 24 hours, NIH/3T3 treated with 100 mM Lactate sodium for 24 hours

  • 纯化方式

    Protein A

  • 浓度

    0.5 mg/ml

  • 标记

    Unconjugated

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

  • 应用

    IHC-P ?

    ICC ?

    WB

  • 稀释度

    应用 稀释度 推荐种属
    WB 1:1000-1:2000 Hu, Ms
    IHC-P 1:500 Hu
    ICC 1:500 Hu
背景介绍

  • Histone H4 (Lactyl K5), denoted as H4K5la, is a specific post-translational modification where a lactyl group derived from cellular lactate is covalently attached to the 5th lysine residue of the histone H4 N-terminal tail. As a critical component of the recently discovered "histone lactylation" code, this mark functions as a direct epigenetic sensor linking high glycolytic flux and lactate accumulation to chromatin regulation. Unlike the well-studied acetylation at the same site (H4K5ac), which is generally associated with active transcription and nucleosome assembly during DNA replication, H4K5la has been increasingly implicated in distinct pathological and physiological contexts, such as the pro-inflammatory activation of microglia following ischemic stroke, where it promotes the transcription of key immunometabolic genes (e.g., HK1, Fads2) to drive fatty acid-dependent inflammatory responses. The dynamic deposition of H4K5la, potentially regulated by specific writers like GCN5 in response to metabolic stress, establishes a unique mechanistic bridge between cellular metabolism (particularly the Warburg effect or hypoxia-induced glycolysis) and the fine-tuning of gene expression programs involved in inflammation, tissue injury, and potentially tumor progression, offering novel therapeutic targets for diseases characterized by metabolic-epigenetic dysregulation.

  • 免疫印迹

    • WB result of Histone H4 (Lactyl K5) Recombinant Rabbit mAb
      Primary antibody: Histone H4 (Lactyl K5) Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: untreated HeLa whole cell lysate 20 µg
      Lane 2: HeLa treated with 100 mM Lactate sodium for 24 hours whole cell lysate 20 µg
      Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 11 kDa
      Observed MW: 14 kDa

    • WB result of Histone H4 (Lactyl K5) Recombinant Rabbit mAb
      Primary antibody: Histone H4 (Lactyl K5) Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: untreated NIH/3T3 whole cell lysate 20 µg
      Lane 2: NIH/3T3 treated with 100 mM Lactate sodium for 24 hours whole cell lysate 20 µg
      Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 11 kDa
      Observed MW: 14 kDa

  • 斑点杂交

    • Dot blot result of Histone H4 (Lactyl K5) Recombinant Rabbit mAb
      Lane 1: Histone H4 (Lactyl K5) modified peptide
      Lane 2: Histone H4 (Lactyl K5) unmodified peptide control
      Primary antibody: Histone H4 (Lactyl K5) Recombinant Rabbit mAb at 1/1000 dilution
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution

  • 免疫组化

    • IHC shows positive staining in paraffin-embedded human tonsil. Anti-Histone H4 (Lactyl K5) antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human lung squamous cell carcinoma. Anti-Histone H4 (Lactyl K5) antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human prostatic cancer. Anti-Histone H4 (Lactyl K5) antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

  • 免疫细胞化学

    • ICC analysis of HeLa cells treated with Lactate sodium (100 mM, 24 hours) (top panel) and untreated HeLa cells (below panel). Anti-Histone H4 (Lactyl K5) antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).

  • 组织表达图谱

    • Expression of Histone H4 (Lactyl K5) in tumor tissues.

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