产品中心 蛋白翻译后修饰(PTM)抗体 位点特异性修饰抗体
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宿主来源
Rabbit抗原名称
Histone H3 (Lactyl K18)分子别名
H3K18la免疫原
Synthetic Peptide细胞定位
NucleusAccession
P68431克隆号
S-3217-72抗体类型
Recombinant mAb抗体同种型
IgG翻译后修饰类型
乳酸化反应种属 ?
Hu, Ms, Rt阳性样本
HeLa treated with 100 mM Lactate sodium for 24 hours, NIH/3T3 treated with 100 mM Lactate sodium for 24 hours纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
ChIP
ICC ?
WB
稀释度
应用 稀释度 推荐种属 Dot Blot 1:1000 WB 1:1000-1:2000 Hu, Ms ICC 1:500 Hu, Ms ChIP 1:20-1:50 Hu
Histone H3 (Lactyl K18) refers to a specific post-translational modification on the 18th lysine residue (K18) of the histone H3 protein, where a lactate-derived lactyl group is covalently attached. Discovered as part of the broader "histone lactylation" mechanism, this epigenetic mark serves as a direct metabolic sensor that links cellular glycolysis and lactate production to gene regulation. Unlike traditional acetylation, which is often associated with general transcriptional activation, H3K18 lactylation (H3K18la) is particularly enriched during specific physiological states such as the late phase of macrophage polarization (driving the transition from pro-inflammatory M1 to reparative M2 phenotypes), hypoxia, and intense exercise. By recruiting specific reader proteins or altering chromatin accessibility at promoter and enhancer regions, H3K18la facilitates the expression of genes involved in tissue repair, angiogenesis, and metabolic adaptation, thereby establishing a crucial mechanistic bridge between cellular metabolism (the Warburg effect or high glycolytic flux) and the epigenetic control of cell fate and function.
免疫印迹
WB result of Histone H3 (Lactyl K18) Recombinant Rabbit mAb
Primary antibody: Histone H3 (Lactyl K18) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated HeLa whole cell lysate 20 µg
Lane 2: HeLa treated with 100 mM Lactate sodium for 24 hours whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 15 kDa
Observed MW: 17 kDaWB result of Histone H3 (Lactyl K18) Recombinant Rabbit mAb
Primary antibody: Histone H3 (Lactyl K18) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated NIH/3T3 whole cell lysate 20 µg
Lane 2: NIH/3T3 treated with 100 mM Lactate sodium for 24 hours whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 15 kDa
Observed MW: 17 kDa
斑点杂交
Dot blot result of Histone H3 (Lactyl K18) Recombinant Rabbit mAb
Lane 1: Histone H3K18 lactoylation peptide
Lane 2: Histone H3 unmodified peptide
Primary antibody: Histone H3 (Lactyl K18) Recombinant Rabbit mAb at 1/1000 dilution
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
免疫细胞化学
ICC analysis of HeLa cells treated with Lactate sodium (100 mM, 24 hours) (top panel) and untreated HeLa cells (below panel). Anti-Histone H3 (Lactyl K18) antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
ICC analysis of NIH/3T3 cells treated with Lactate sodium (100 mM, 24 hours) (top panel) and untreated NIH/3T3 cells (below panel). Anti-Histone H3 (Lactyl K18) antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
ChIP
Chromatin immunoprecipitation (ChIP) was performed on HeLa cells cross-linked with 1% formaldehyde for 10 min, then chromatin was fragmented by sonication. Parallel reactions used Histone H3 (Lactyl K18) Recombinant Rabbit mAb (S-3217-72) and Rabbit mAb IgG Isotype Control (SDT-R173) at 1:50 for immunoprecipitation.
Post-immunoprecipitation, both samples were washed, eluted, and cross-links reversed. Purified DNA was analyzed by qPCR.
qPCR
showed the enrichment of RPL30, GAPDH, MYOD1,
AFM, SAT-α and SAT-2 in Histone H3 (Lactyl K18)
Recombinant Rabbit mAb (S-3217-72)-immunoprecipitated
sample.
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