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MAPKAPK-2 Recombinant Rabbit mAb (S-2641-58)

MAP kinase-activated protein kinase 2,MAPK-activated protein kinase 2,MAPKAP kinase 2,MAPKAP-K2,MK-2,MK2,MAPKAPK2

价格 600.00 供应商现货 : 3-5个工作日
货号 S0B6741
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产品介绍 评论(0)

产品规格
  • 宿主来源

    Rabbit
  • 抗原名称

    MAPKAPK-2
  • 分子别名

    MAP kinase-activated protein kinase 2; MAPK-activated protein kinase 2; MAPKAP kinase 2; MAPKAP-K2; MK-2; MK2; MAPKAPK2
  • 免疫原

    Synthetic Peptide
  • 细胞定位

    Cytoplasm, Nucleus
  • Accession

    P49137
  • 克隆号

    S-2641-58
  • 抗体类型

    Recombinant mAb
  • 抗体同种型

    IgG
  • 反应种属 ?

    Hu, Ms, Rt, Mk
  • 阳性样本

    HeLa treated with UV (100 mJ/cm2, 1 hour recovery), 293T, A431, NIH/3T3, MEF, mouse brain, mouse spleen, C6, rat brain, rat spleen, COS-7
  • 预测反应种属
    (反应种属缩写表)

    Hm, Rb
  • 纯化方式

    Protein A
  • 浓度

    0.5 mg/ml
  • 标记

    Unconjugated
  • 性状

    Liquid
  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

  • 应用

    WB

  • 稀释度

    应用 稀释度 推荐种属
    WB 1:1000-1:2000 Hu, Ms, Rt, Mk
背景介绍
  • MAPKAPK-2 (commonly referred to as MK2) is a serine/threonine protein kinase encoded by the MAPKAPK2 gene and serves as the most pivotal direct substrate of p38 MAPK, acting as a signaling hub in inflammation, tumorigenesis, the DNA damage response, and cellular senescence. In its resting state, MK2 remains inactive due to an autoinhibitory conformation formed between its C-terminal regulatory domain and its kinase domain. Upon exposure to cellular stress, inflammatory cytokines, or pathogen stimulation, p38α phosphorylates MK2 at Thr334, triggering a conformational switch that simultaneously exposes a nuclear export signal (NES) —promoting p38-bound MK2 translocation to the cytoplasm—and masks its C-terminal nuclear localization signal (NLS) , thereby directing p38 signaling precisely toward cytoplasmic targets. Activated MK2 executes pleiotropic functions via phosphorylation of a diverse substrate repertoire: in the cytoplasm, it phosphorylates HSP27 to remodel the actin cytoskeleton and facilitate cell migration; in RNA metabolism, it phosphorylates ARE-binding proteins such as Tristetraprolin (TTP) and hnRNP A0, causing their 14-3-3-mediated sequestration and inactivation, thereby stabilizing the mRNAs of pro-inflammatory cytokines including TNF-α, IL-6, and IL-1β—a core mechanism driving chronic inflammation. Within the nucleus, MK2 phosphorylates transcription factors such as CREB and SRF, and contributes to maintaining the G2/M checkpoint by regulating the stability of GADD45A mRNA following DNA damage. Recent studies have expanded the functional landscape of MK2 far beyond the classical inflammatory axis: within the tumor microenvironment, MK2 promotes cell survival, drives epithelial-mesenchymal transition (EMT), and confers chemoresistance (e.g., desensitizing pancreatic cancer to gemcitabine and tongue squamous cell carcinoma to paclitaxel), positioning it as a promising target for overcoming drug resistance. In senescent cells, the mTOR pathway selectively upregulates MK2 translation via 4EBP1; the accumulated MK2 then phosphorylates ZFP36L1, relieving its degradation of SASP component mRNAs and thereby establishing the senescence-associated secretory phenotype (SASP).

  • 免疫印迹

    • WB result of MAPKAPK-2 Recombinant Rabbit mAb
      Primary antibody: MAPKAPK-2 Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: untreated HeLa whole cell lysate 20 µg
      Lane 2: HeLa treated with UV (100 mJ/cm2, 1 hour recovery) whole cell lysate 20 µg
      Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 45 kDa
      Observed MW: 45, 56 kDa

    • WB result of MAPKAPK-2 Recombinant Rabbit mAb
      Primary antibody: MAPKAPK-2 Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: 293T whole cell lysate 20 µg
      Lane 2: A431 whole cell lysate 20 µg
      Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 45 kDa
      Observed MW: 45, 56 kDa

    • WB result of MAPKAPK-2 Recombinant Rabbit mAb
      Primary antibody: MAPKAPK-2 Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: NIH/3T3 whole cell lysate 20 µg
      Lane 2: MEF whole cell lysate 20 µg
      Lane 3: mouse brain lysate 20 µg
      Lane 4: mouse spleen lysate 20 µg
      Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 45 kDa
      Observed MW: 40, 49 kDa

    • WB result of MAPKAPK-2 Recombinant Rabbit mAb
      Primary antibody: MAPKAPK-2 Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: C6 whole cell lysate 20 µg
      Lane 2: rat brain lysate 20 µg
      Lane 3: rat spleen lysate 20 µg
      Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 45 kDa
      Observed MW: 40, 49 kDa

    • WB result of MAPKAPK-2 Recombinant Rabbit mAb
      Primary antibody: MAPKAPK-2 Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: COS-7 whole cell lysate 20 µg
      Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 45 kDa
      Observed MW: 45, 55 kDa

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