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Rabbit抗原名称
Pericentrin分子别名
Kendrin; Pericentrin-B; KIAA0402; PCNT2; PCNT免疫原
Recombinant Protein细胞定位
Centrosome, Cytoplasm, CytoskeletonAccession
O95613克隆号
S-3515-69抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt, Mk阳性样本
HEK293, HepG2, HeLa, MCF7, NIH/3T3, mouse testis, C6, rat testis, COS-7纯化方式
Protein A浓度
2 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
ICC ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu, Ms, Rt, MK ICC 1:1000-1:2000 Hu
Pericentrin (PCNT, also called kendrin or pericentrin-B) is a large, highly conserved coiled-coil scaffold protein encoded by the PCNT gene on chromosome 21, which localizes to the centrosome and pericentriolar material (PCM) via its C-terminal PACT domain, anchoring γ-tubulin complexes and other regulatory proteins to organize microtubule nucleation and anchoring, thereby orchestrating centrosome assembly, mitotic spindle formation, cell-cycle progression and primary cilium biogenesis; mutations or dysregulation of PCNT underlie disorders such as microcephalic osteodysplastic primordial dwarfism type II, Seckel syndrome, ciliopathies, and have been linked to cancer, mental disorders and altered insulin secretion in β-cells.
免疫印迹
WB result of Pericentrin Recombinant Rabbit mAb
Primary antibody: Pericentrin Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HEK-293 whole cell lysate 20 µg
Lane 2: HepG2 whole cell lysate 20 µg
Lane 3: HeLa whole cell lysate 20 µg
Lane 4: MCF7 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 378 kDa
Observed MW: 180 kDa
This blot was developed with high sensitivity substrateWB result of Pericentrin Recombinant Rabbit mAb
Primary antibody: Pericentrin Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: mouse testis lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 378 kDa
Observed MW: 180 kDaWB result of Pericentrin Recombinant Rabbit mAb
Primary antibody: Pericentrin Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: C6 whole cell lysate 20 µg
Lane 2: rat testis lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 378 kDa
Observed MW: 180 kDaWB result of Pericentrin Recombinant Rabbit mAb
Primary antibody: Pericentrin Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 378 kDa
Observed MW: 180 kDa
This blot was developed with high sensitivity substrate
免疫细胞化学
ICC shows positive staining in HeLa cells. Anti-Pericentrin antibody was used at 1/2000 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
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