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Rabbit抗原名称
NNMT分子别名
Nicotinamide N-methyltransferase免疫原
Recombinant Protein细胞定位
CytoplasmAccession
P40261克隆号
S-3470-58抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu阳性样本
A549, HeLa, HUVEC,纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu IHC-P 1:2000 Hu
Nicotinamide N-methyltransferase (NNMT) is a cytosolic metabolic enzyme encoded by a gene located on chromosome 11q23.2. Its core biochemical function is the transfer of a methyl group from S-adenosylmethionine (SAM) to nicotinamide (NAM), generating 1-methylnicotinamide (1-MNA) and S-adenosylhomocysteine (SAH). This reaction positions NNMT as a pivotal nexus linking methylation metabolism, NAD⁺ synthesis, and epigenetic regulation. NNMT's sustained consumption of the SAM substrate not only limits methyl donor availability for DNA methyltransferases and histone methyltransferases—leading to loss of repressive histone marks such as H3K27me3 and global DNA hypomethylation—but also depletes the NAD⁺ precursor nicotinamide, thereby impairing mitochondrial respiration and the activity of deacetylases such as SIRT1. This accelerates mitochondrial dysfunction and neuronal apoptosis in neurodegenerative diseases, while within the tumor microenvironment, it drives the activation and differentiation of cancer-associated fibroblasts (CAFs) and recruits myeloid-derived suppressor cells (MDSCs) via the complement cascade to establish an immunosuppressive niche. Research has confirmed that NNMT is specifically upregulated by the glucocorticoid receptor signaling axis in glioblastoma, where its product 1-MNA is enriched approximately 7-fold in tumor tissue compared to adjacent normal tissue—a disparity now exploited for [¹¹C] nicotinamide PET imaging strategies. In hypertrophic scars, NNMT-mediated NAD⁺ and SAM depletion drives macrophage-to-myofibroblast transdifferentiation (MMT) through accumulation of H3K27ac and loss of H3K27me3; NNMT knockdown disrupts the interaction between the transcription factor Prrx1 and super-enhancers, significantly reducing scar volume.
免疫印迹
WB result of NNMT Recombinant Rabbit mAb
Primary antibody: NNMT Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: PANC-1 whole cell lysate 20 µg
Lane 2: A549 whole cell lysate 20 µg
Lane 3: HeLa whole cell lysate 20 µg
Lane 4: HUVEC whole cell lysate 20 µg
Negative control: PANC-1 whole cell lysate
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 29 kDa
Observed MW: 25 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human colon. Anti-NNMT antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human liver. Anti-NNMT antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human spleen. Anti-NNMT antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-NNMT antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human ovarian cancer. Anti-NNMT antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human cerebral cortex. Anti-NNMT antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human skeletal muscle. Anti-NNMT antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of NNMT in tumor tissues.
Expression of NNMT in human tissues.
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