TGN46 Recombinant Rabbit mAb (S-3514-47)
Trans-Golgi network integral membrane protein 2,Trans-Golgi network glycoprotein 46 (TGN38 homolog,hTGN46),Trans-Golgi network glycoprotein 48 (hTGN48),Trans-Golgi network glycoprotein 51 (hTGN51),Trans-Golgi network protein 2Imported,TGN51,TGOLN2
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宿主来源
Rabbit抗原名称
TGN46分子别名
Trans-Golgi network integral membrane protein 2; Trans-Golgi network glycoprotein 46 (TGN38 homolog; hTGN46); Trans-Golgi network glycoprotein 48 (hTGN48); Trans-Golgi network glycoprotein 51 (hTGN51); Trans-Golgi network protein 2Imported; TGN51; TGOLN2免疫原
Recombinant Protein细胞定位
Endosome, Cell membraneAccession
O43493克隆号
S-3514-47抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu阳性样本
HepG2, A549, A172纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
ICC ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu IHC-P 1:1000 Hu ICC 1:500 Hu
TGN46 (Trans-Golgi Network protein 46 kDa) is a type I integral membrane protein predominantly localized to the trans-Golgi network (TGN), where it functions as a critical component of the protein sorting and vesicular trafficking machinery. This 46-kilodalton protein features a large N-terminal lumenal domain containing multiple N-glycosylation sites, a single transmembrane helix, and a short C-terminal cytoplasmic tail harboring sorting signals—including tyrosine-based motifs (YXXΦ)—that facilitate its interaction with adaptor protein complexes (AP-1 and AP-2) to regulate cargo selection and vesicle formation. TGN46 serves as a widely used marker for the TGN in immunofluorescence and cell biology studies, and plays essential roles in maintaining TGN structure, recycling mannose-6-phosphate receptors (MPRs) between endosomes and the Golgi, and sorting lysosomal enzymes into clathrin-coated vesicles destined for late endosomes. Its dynamic cycling between the TGN, endosomes, and the plasma membrane—mediated by phosphorylation-dependent conformational changes and interactions with various coat proteins—underscores its significance in secretory and endocytic pathway regulation, making it indispensable for proper cellular homeostasis and protein trafficking fidelity.
免疫印迹
WB result of TGN46 Recombinant Rabbit mAb
Primary antibody: TGN46 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: HepG2 whole cell lysate 20 µg
Lane 3: A549 whole cell lysate 20 µg
Lane 4: A172 whole cell lysate 20 µg
Low expression control: HeLa whole cell lysate
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 46 kDa
Observed MW: 75-100 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-TGN46 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon. Anti-TGN46 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human kidney. Anti-TGN46 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-TGN46 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung cancer. Anti-TGN46 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
ICC shows positive staining in A549 cells. Anti-TGN46 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
组织表达图谱
Expression of TGN46 in human tissues.
Expression of TGN46 in tumor tissues.
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