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Rabbit抗原名称
PINK1分子别名
Serine/threonine-protein kinase PINK1, mitochondrial; BRPK; PTEN-induced putative kinase protein 1免疫原
Synthetic Peptide细胞定位
Cytoplasm, MitochondrionAccession
Q9BXM7克隆号
S-3135-28抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu阳性样本
CCCP treated HeLa, CCCP treated HEK-293纯化方式
Protein A浓度
2 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu
PINK1 (PTEN-induced putative kinase 1) is a serine/threonine kinase primarily localized to mitochondria that serves as a critical sensor for mitochondrial quality control through the PINK1/Parkin mitophagy pathway; upon mitochondrial damage, PINK1 stabilizes on the outer mitochondrial membrane and phosphorylates both ubiquitin and the E3 ubiquitin ligase Parkin, initiating the selective autophagic clearance of dysfunctional mitochondria, while loss-of-function mutations in the PINK1 gene are linked to autosomal recessive early-onset Parkinson's disease, highlighting its essential role in neuronal survival and protection against neurodegeneration.
免疫印迹
WB result of PINK1 Recombinant Rabbit mAb
Primary antibody: PINK1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated HeLa whole cell lysate 20 µg
Lane 2: HeLa treated with 10 µM CCCP for 24 hours whole cell lysate 20 µg
Lane 3: untreated HEK-293 whole cell lysate 20 µg
Lane 4: HEK-293 treated with 30 µM CCCP for 6 hours whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 63 kDa
Observed MW: 63 kDa
This blot was developed with high sensitivity substrate
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