分享产品介绍 评论(0)
宿主来源
Rabbit抗原名称
Olfactory Marker/OMP分子别名
Olfactory marker protein; Olfactory neuronal-specific protein; OMP免疫原
Recombinant Protein细胞定位
CytoplasmAccession
P47874克隆号
S-3513-32抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Ms, Rt阳性样本
mouse olfactory bulb, rat olfactory bulb纯化方式
Protein A浓度
0.25 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
IF ?
稀释度
应用 稀释度 推荐种属 WB 1:1000 Ms, Rt IHC-P 1:1000 Ms, Rt IF 1:250 Ms, Rt
Olfactory Marker Protein (OMP) is a small, ~19 kDa cytosolic protein uniquely and abundantly expressed in mature olfactory receptor neurons (ORNs) across the vertebrate olfactory epithelium, from the cell soma to the distal axon terminals in the olfactory bulb, where it was first discovered by Frank Margolis in the 1970s as a specific molecular signature for terminally differentiated olfactory neurons. Although its precise physiological function remains incompletely understood, OMP is believed to modulate olfactory signal transduction by interacting with odorant receptor signaling cascades, regulating cAMP kinetics, and potentially facilitating odorant adaptation, while also playing roles in axon guidance, synaptic targeting, and the refinement of olfactory maps during development and regeneration. As the definitive immunohistochemical marker for mature ORNs, OMP distinguishes fully differentiated neurons from immature (expressing GAP43) and sustentacular cells, making it indispensable for studying neuronal turnover, lesion-induced regeneration, and the functional anatomy of the olfactory system in both normal and pathological states, including neurodegenerative diseases and post-viral olfactory dysfunction.
免疫印迹
WB result of Olfactory Marker/OMP Recombinant Rabbit mAb
Primary antibody: Olfactory Marker/OMP Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: RAW264.7 whole cell lysate 20 µg
Lane 3: Neuro-2a whole cell lysate 20 µg
Lane 4: mouse spleen lysate 20 µg
Lane 5: mouse kidney lysate 20 µg
Lane 6: mouse heart lysate 20 µg
Lane 7: mouse brain lysate 20 µg
Lane 8: mouse olfactory bulb lysate 20 µg
Negative control: NIH/3T3 whole cell lysate; RAW264.7 whole cell lysate; Neuro-2a whole cell lysate; mouse spleen lysate; mouse kidney lysate; mouse heart lysate; mouse brain lysate
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 19 kDa
Observed MW: 21 kDaWB result of Olfactory Marker/OMP Recombinant Rabbit mAb
Primary antibody: Olfactory Marker/OMP Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: PC-12 whole cell lysate 20 µg
Lane 2: C6 whole cell lysate 20 µg
Lane 3: rat kidney lysate 20 µg
Lane 4: rat olfactory bulb lysate 20 µg
Negative control: PC-12 whole cell lysate; C6 whole cell lysate; rat kidney lysate
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 19 kDa
Observed MW: 21 kDa
免疫组化
IHC shows positive staining in paraffin-embedded mouse olfactory bulb. Anti-Olfactory marker/OMP antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded mouse spleen. Anti-Olfactory marker/OMP antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat olfactory bulb. Anti-Olfactory marker/OMP antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded rat cerebral cortex. Anti-Olfactory marker/OMP antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫荧光
IF shows positive staining in paraffin-embedded mouse olfactory bulb. Anti-Olfactory Marker/OMP antibody was used at 1/250 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows negative staining in paraffin-embedded mouse cerebral cortex. Anti-Olfactory Marker/OMP antibody was used at 1/250 dilution and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows positive staining in paraffin-embedded rat olfactory bulb. Anti-Olfactory Marker/OMP antibody was used at 1/250 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows negative staining in paraffin-embedded rat cerebral cortex. Anti-Olfactory Marker/OMP antibody was used at 1/250 dilution and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
组织表达图谱
Expression of Olfactory marker/OMP in mouse & rat tissues.
评论(0)