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宿主来源
Rabbit抗原名称
MTCO2分子别名
Cytochrome c oxidase subunit 2; Cytochrome c oxidase polypeptide II; COII; COX2; COXII; MTCO2Encoded inMitochondrion; MT-CO2免疫原
Synthetic Peptide细胞定位
MitochondrionAccession
P00403克隆号
S-1891-72抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu阳性样本
HeLa, MCF7, K562, COLO 205, THP-1纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20°C as supplied
应用
IHC-P ?
ICC ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000-1:5000 Hu IHC-P 1:1000 Hu ICC 1:50-1:100 Hu
MTCO2 (Mitochondrially Encoded Cytochrome C Oxidase II, also known as COX2 or COII) is a ~25 kDa protein encoded by the mitochondrial genome and embedded in the inner mitochondrial membrane as one of the three mitochondrially-encoded core subunits of cytochrome c oxidase (Complex IV), the terminal enzyme complex of the electron transport chain. This protein plays a critical role in aerobic respiration by mediating the transfer of electrons from cytochrome c to molecular oxygen via its binuclear copper A center (CuA), ultimately catalyzing the reduction of oxygen to water while contributing to the generation of the proton gradient essential for ATP synthesis through oxidative phosphorylation. Highly expressed in energy-demanding tissues such as cardiac muscle, skeletal muscle, and neurons, MTCO2 functions as a pivotal component of the respiratory chain, and its mutations or dysfunctions have been implicated in various mitochondrial disorders, including mitochondrial myopathy, Leber's hereditary optic neuropathy, and other conditions arising from compromised oxidative phosphorylation and cellular energy failure.
免疫印迹
WB result of MTCO2 Recombinant Rabbit mAb
Primary antibody: MTCO2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: MCF7 whole cell lysate 20 µg
Lane 3: K562 whole cell lysate 20 µg
Lane 4: COLO 205 whole cell lysate 20 µg
Lane 5: THP-1 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 25 kDa
Observed MW: 24 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human kidney. Anti-MTCO2 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human liver. Anti-MTCO2 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-MTCO2 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human hepatocellular carcinoma. Anti-MTCO2 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
ICC shows positive staining in HeLa cells. Anti-MTCO2 antibody was used at 1/50 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
组织表达图谱
Expression of MTCO2 in human tissues.
Expression of MTCO2 in tumor tissues.
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