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BCAR1 Recombinant Rabbit mAb (S-3542-49)

Breast cancer anti-estrogen resistance protein 1,CRK-associated substrate,Cas scaffolding protein family member 1,p130cas,CAS,CASS1,CRKAS

价格 ￥600.00 （ 供应商现货 ： 3-5个工作日）
货号 S0B6717
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产品介绍

产品介绍 引用文献(1) 评论(0)

产品规格

  • 宿主来源

    Rabbit

  • 抗原名称

    BCAR1

  • 分子别名

    Breast cancer anti-estrogen resistance protein 1; CRK-associated substrate; Cas scaffolding protein family member 1; p130cas; CAS; CASS1; CRKAS

  • 免疫原

    Recombinant Protein

  • 细胞定位

    Cytoplasm

  • Accession

    P56945

  • 克隆号

    S-3542-49

  • 抗体类型

    Recombinant mAb

  • 抗体同种型

    IgG

  • 反应种属 ?

    Hu, Ms, Rt

  • 阳性样本

    HeLa, HepG2, A431, NIH/3T3, C6

  • 纯化方式

    Protein A

  • 浓度

    0.5 mg/ml

  • 标记

    Unconjugated

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

  • 应用

    IHC-P ?

    ICC ?

    WB

  • 稀释度

    应用 稀释度 推荐种属
    WB 1:1000 Hu, Ms, Rt
    IHC-P 1:200 Hu, Ms, Rt
    ICC 1:500 Hu, Ms
背景介绍

  • BCAR1 (Breast Cancer Anti-Estrogen Resistance 1), also known as p130Cas (Crk-associated substrate), is a multifunctional adaptor/scaffold protein that plays a pivotal role in integrating signals from integrins, growth factor receptors, and other cellular stimuli to regulate cell adhesion, migration, proliferation, and survival. Structurally, BCAR1 contains an N-terminal SH3 domain, a proline-rich region, a large substrate domain with multiple YxxP motifs that serve as phosphorylation sites for Src family kinases, and a C-terminal focal adhesion targeting (FAT) domain that mediates its localization to focal adhesions through interactions with paxillin and other proteins. Upon tyrosine phosphorylation, BCAR1 acts as a docking platform for various signaling molecules including Crk, Nck, and PI3K, thereby activating downstream pathways such as Ras-MAPK and PI3K-AKT that promote cytoskeletal reorganization and oncogenic transformation. Aberrant overexpression or hyperactivation of BCAR1 has been strongly implicated in tumor progression, metastasis, and resistance to endocrine therapies in breast cancer and other malignancies, making it a potential prognostic biomarker and therapeutic target.

  • 免疫印迹

    • WB result of BCAR1 Recombinant Rabbit mAb
      Primary antibody: BCAR1 Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: Molt-4 whole cell lysate 20 µg
      Lane 2: Jurkat whole cell lysate 20 µg
      Lane 3: HeLa whole cell lysate 20 µg
      Lane 4: HepG2 whole cell lysate 20 µg
      Lane 5: A431 whole cell lysate 20 µg
      Negative control: Molt-4 whole cell lysate
      Low expression control: Jurkat whole cell lysate
      Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 93 kDa
      Observed MW: 120 kDa

    • WB result of BCAR1 Recombinant Rabbit mAb
      Primary antibody: BCAR1 Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: NIH/3T3 whole cell lysate 20 µg
      Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 93 kDa
      Observed MW: 120 kDa

    • WB result of BCAR1 Recombinant Rabbit mAb
      Primary antibody: BCAR1 Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: C6 whole cell lysate 20 µg
      Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 93 kDa
      Observed MW: 120 kDa

  • 免疫组化

    • IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-BCAR1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human colon. Anti-BCAR1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human liver. Anti-BCAR1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human testis. Anti-BCAR1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human colon cancer. Anti-BCAR1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-BCAR1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded mouse colon. Anti-BCAR1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti-BCAR1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

  • 免疫细胞化学

    • ICC shows positive staining in HeLa cells. Anti-BCAR1 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).

    • ICC shows positive staining in NIH/3T3 cells. Anti-BCAR1 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).

  • 组织表达图谱

    • Expression of BCAR1 in human tissues.

    • Expression of BCAR1 in tumor tissues.

    • Expression of BCAR1 in mouse & rat tissues.

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