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Rabbit抗原名称
ATR分子别名
Serine/threonine-protein kinase ATR; Ataxia telangiectasia and Rad3-related protein; FRAP-related protein 1; FRP1免疫原
Synthetic Peptide细胞定位
NucleusAccession
Q13535克隆号
S-3506-59抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu阳性样本
Caco-2, A431, HeLa, HT-29纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu
ATR (Ataxia Telangiectasia and Rad3-related) protein is a serine/threonine kinase that serves as a master regulator of the DNA damage response (DDR), particularly in response to replication stress and DNA double-strand breaks; it is recruited to sites of DNA damage through its interaction with RPA-coated single-stranded DNA (ssDNA) and the Rad9-Rad1-Hus1 (9-1-1) clamp complex, whereupon it phosphorylates key downstream substrates including the checkpoint kinase Chk1, p53, BRCA1, and RAD51 to initiate cell cycle arrest, facilitate DNA repair, and prevent replication fork collapse, thereby maintaining genomic integrity and preventing tumorigenesis, with its dysregulation being implicated in various cancers and its inhibition emerging as a promising therapeutic strategy for cancer treatment.
免疫印迹
WB result of ATR Recombinant Rabbit mAb
Primary antibody: ATR Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: Caco-2 whole cell lysate 20 µg
Lane 2: A431 whole cell lysate 20 µg
Lane 3: HeLa whole cell lysate 20 µg
Lane 4: HT-29 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 301 kDa
Observed MW: 280 kDa
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