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NFIB / NF1B2 Recombinant Rabbit mAb (S-3046-78)

Nuclear factor 1 B-type,NF1-B,Nuclear factor 1/B,CCAAT-box-binding transcription factor (CTF),Nuclear factor I/B (NF-I/B,NFI-B),TGGCA-binding protein

价格 600.00 供应商现货 : 3-5个工作日
货号 S0B6712
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产品规格
  • 宿主来源

    Rabbit
  • 抗原名称

    NFIB / NF1B2
  • 分子别名

    Nuclear factor 1 B-type; NF1-B; Nuclear factor 1/B; CCAAT-box-binding transcription factor (CTF); Nuclear factor I/B (NF-I/B; NFI-B); TGGCA-binding protein
  • 免疫原

    Synthetic Peptide
  • 细胞定位

    Nucleus
  • Accession

    O00712
  • 克隆号

    S-3046-78
  • 抗体类型

    Recombinant mAb
  • 抗体同种型

    IgG
  • 反应种属 ?

    Hu, Ms, Rt
  • 阳性样本

    RPMI-8226, MCF7, HeLa, NIH/3T3
  • 预测反应种属
    (反应种属缩写表)

    Bv, Hm
  • 纯化方式

    Protein A
  • 浓度

    0.5 mg/ml
  • 标记

    Unconjugated
  • 性状

    Liquid
  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

  • 应用

    WB

  • 稀释度

    应用 稀释度 推荐种属
    WB 1:1000 Hu, Ms, Rt
背景介绍
  • The NFIB protein, also known as Nuclear Factor I B or NF1B2, is a transcription factor belonging to the NFI (Nuclear Factor I) family of site-specific DNA-binding proteins that play crucial roles in regulating gene expression during development and cellular differentiation. Encoded by the NFIB gene located on human chromosome 9p23-p22, this protein contains a highly conserved N-terminal DNA-binding domain that recognizes the palindromic consensus sequence 5'-TTGGC(N)5GCCAA-3', thereby controlling the transcription of target genes involved in various biological processes including brain development, lung maturation, and skeletal patterning. NFIB functions as both a transcriptional activator and repressor depending on cellular context and promoter architecture, and it forms homodimers or heterodimers with other NFI family members (NFIA, NFIC, and NFID) to exert its regulatory effects. During embryonic development, NFIB is essential for proper formation of the corpus callosum, cerebellar vermis, and facial structures, while in the postnatal period it regulates terminal differentiation of various cell types including chondrocytes, osteoblasts, and respiratory epithelial cells. Aberrant NFIB expression or function has been implicated in multiple pathological conditions, including certain forms of intellectual disability, craniofacial abnormalities, and various cancers where it can act as either an oncogene or tumor suppressor depending on the tissue type. The protein's activity is modulated through post-translational modifications and interactions with various co-activators and co-repressors, making it a critical node in the gene regulatory networks that govern mammalian development and homeostasis.

  • 免疫印迹

    • WB result of NFIB/NF1B2 Recombinant Rabbit mAb
      Primary antibody: NFIB/NF1B2 Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: RPMI-8226 whole cell lysate 20 µg
      Lane 2: MCF7 whole cell lysate 20 µg
      Lane 3: HeLa whole cell lysate 20 µg
      Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 47 kDa
      Observed MW: 45-70 kDa

    • WB result of NFIB/NF1B2 Recombinant Rabbit mAb
      Primary antibody: NFIB / NF1B2 Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: NIH/3T3 whole cell lysate 20 µg
      Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 47 kDa
      Observed MW: 45-70 kDa

    • WB result of NFIB/NF1B2 Recombinant Rabbit mAb
      Primary antibody: NFIB/NF1B2 Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: C6 whole cell lysate 20 µg
      Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 47 kDa
      Observed MW: 45-70 kDa

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