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Rabbit抗原名称
53BP1分子别名
TP53-binding protein 1; 53BP1; p53-binding protein 1; p53BP1; TP53BP1免疫原
Synthetic Peptide细胞定位
NucleusAccession
Q12888克隆号
S-3170-149抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu阳性样本
HT-29, HCT 116纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
ICC ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu IHC-P 1:1000 Hu ICC 1:500 Hu
53BP1 (p53-binding protein 1) is a large, multi-domain scaffold protein that rapidly accumulates in irradiation-induced foci at DNA double-strand breaks, where it acts as a master regulator of the non-homologous end-joining repair pathway by binding phosphorylated H2AX (γH2AX) and histone H4 methylated on Lys20, thereby recruiting the downstream effector kinase ATM, the ubiquitin ligases RNF8/RNF168, and the adaptor proteins BRCA1 and RIF1 to antagonize BRCA1-mediated DNA-end resection and channel repair toward the error-prone but fast NHEJ route, while its own recruitment and retention are governed by tandem Tudor and BRCT domains that sense combinatorial histone marks, and its loss or mutation leads to genomic instability, hypersensitivity to ionizing radiation, and impaired class-switch recombination in B lymphocytes.
免疫印迹
WB result of 53BP1 Recombinant Rabbit mAb
Primary antibody: 53BP1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HT-29 whole cell lysate 20 µg
Lane 2: HCT 116 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 214 kDa
Observed MW: 350 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human tonsil. Anti-53BP1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon. Anti-53BP1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-53BP1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human breast cancer. Anti-53BP1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
ICC shows positive staining in HCT116 cells. Anti-53BP1 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
组织表达图谱
Expression of 53BP1 in tumor tissue.
Expression of 53BP1 in human tissue.
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