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Rabbit抗原名称
ATP7B分子别名
Copper-transporting ATPase 2; Copper pump 2; Wilson disease-associated protein; PWD; WC1; WND免疫原
Recombinant Protein细胞定位
Mitochondrion, Cytoplasm, EndosomeAccession
P35670克隆号
S-2827-58抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu阳性样本
HepG2, Caco-2, K562, HEK-293纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu
ATP7B is a P-type ATPase copper-transporting beta polypeptide that acts as the liver-specific efflux pump, using ATP hydrolysis to move cytosolic Cu⁺ across membranes into the trans-Golgi network for incorporation into ceruloplasmin or, when copper is elevated, to vesicles that fuse with the canalicular membrane to excrete excess copper into bile, thereby maintaining systemic copper homeostasis; mutations in ATP7B disable this export, causing toxic hepatic and brain copper accumulation that defines the autosomal-recessive disorder Wilson disease.
免疫印迹
WB result of ATP7B Recombinant Rabbit mAb
Primary antibody: ATP7B Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: unboiled HepG2 whole cell lysate 20 µg
Lane 2: unboiled Caco-2 whole cell lysate 20 µg
Lane 3: unboiled K562 whole cell lysate 20 µg
Lane 4: unboiled HEK-293 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 157 kDa
Observed MW: 180 kDa
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