FbpB/Ag85B Recombinant Rabbit mAb (S-3325-22)
Diacylglycerol acyltransferase/mycolyltransferase Ag85B,DGAT,30 kDa extracellular protein,Acyl-CoA:diacylglycerol acyltransferase,Antigen 85 complex B (85B,Ag85B),Extracellular alpha-antigen,Fibronectin-binding protein B (Fbps B),fbpB
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宿主来源
Rabbit抗原名称
FbpB/Ag85B分子别名
Diacylglycerol acyltransferase/mycolyltransferase Ag85B; DGAT; 30 kDa extracellular protein; Acyl-CoA:diacylglycerol acyltransferase; Antigen 85 complex B (85B; Ag85B); Extracellular alpha-antigen; Fibronectin-binding protein B (Fbps B); fbpB免疫原
Recombinant Protein细胞定位
SecretedAccession
P9WQP0克隆号
S-3325-22抗体类型
Recombinant mAb抗体同种型
IgG纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Mycobacterium tuberculosis
FbpB/Ag85B is a 30 kDa secreted fibronectin-binding mycolyltransferase that catalyzes the transfer of mycolic acids to arabinogalactan and trehalose monomycolates, thereby building trehalose dimycolate (cord factor) and maintaining the integrity of the mycobacterial cell wall, while simultaneously functioning as the immunodominant antigen of the antigen 85 complex that strongly induces Th1-biased cellular and humoral immunity through IFN-γ, IL-2 and antibody production, making it a key adhesin for host-cell attachment and a leading subunit vaccine candidate against tuberculosis.
免疫印迹
WB result of FbpB/Ag85B Recombinant Rabbit mAb
Primary antibody: FbpB/Ag85B Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: fbpC protein 10 ng
Lane 2: fbpA protein 10 ng
Lane 3: fbpB protein 10 ng
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 35 kDa
Observed MW: 40 kDa
This antibody does not cross-react with fbpA and fbpC
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