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Rabbit抗原名称
Phospho-PKA Substrate (RRXST)免疫原
Synthetic Peptide克隆号
S-2623-148抗体类型
Recombinant mAb抗体同种型
IgG翻译后修饰类型
磷酸化反应种属 ?
Hu, Ms阳性样本
starve overnight and Calyculin A treated HeLa, Calyculin A treated NIH/3T3纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
ELISA
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000-1:5000 Hu, Ms
Phospho-PKA Substrate (RRXST) is a recognition motif for substrates specifically phosphorylated by protein kinase A (PKA). Within this motif, serine (S) or threonine (T) serves as the phosphorylation site, flanked on its N-terminal side by two arginine residues (RR). The position immediately following the second arginine can be any amino acid (X). This highly conserved pattern of basic amino acids ensures that PKA can specifically recognize and catalyze the phosphorylation of serine/threonine residues in its substrates, thereby regulating various critical cellular signaling pathways, including glycogen metabolism, gene transcription, and cell proliferation. Specific phospho-antibodies targeting this motif have become essential research tools for detecting PKA activity and its downstream signaling events.
免疫沉淀
WB result of Phospho-PKA Substrate (RRXS*/T*) Recombinant Rabbit mAb
Blocking/Diluting buffer and concentration: 5% NFDM/TBST
Primary antibody: Phospho-PKA Substrate (RRXS*/T*) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated HeLa whole cell lysate 20 µg
Lane 2: HeLa starve overnight, then treated with 100 nM Calyculin A for 30 minutes whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: Multiple
Observed MW: MultipleWB result of Phospho-PKA Substrate (RRXS*/T*) Recombinant Rabbit mAb
Blocking/Diluting buffer and concentration: 5% NFDM/TBST
Primary antibody: Phospho-PKA Substrate (RRXS*/T*) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated NIH/3T3 whole cell lysate 20 µg
Lane 2: NIH/3T3 treated with 100 nM Calyculin A for 30 minutes whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: Multiple
Observed MW: Multiple
ELISA
ELISA binding assay shows Phospho-PKA Substrate (RRXST) Antibody specifically binds to phospho and non-phospho motif peptides.
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